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Recombinant Human Collagen for 3D Bioprinting of Skin Equivalent
A

B

C D

E F

Figure 3. Assessing the rheology and printability of the bioinks. (A) Storage and loss moduli of the bioinks determined by an oscillatory
temperature sweep from 37 to 4°C. (B) Storage and loss moduli of the bioinks determined during photocrosslinking at 37°C. (C)
Compressive modulus of the photocrosslinked hydrogels with varied rhCol concentrations. Significance is indicated with *P < 0.05 and
**P < 0.01, no significance is indicated with ns, n = 3. (D) Filament fusion test of the bioinks (pictures of printed constructs) and plot of
fused filament length (fs) normalized by filament thickness (ft) as a function of the filament distance (fd) of the tested bioinks. (E) LIVE/
DEAD™ fluorescent staining images of the printed HDFs in different bioinks post-photocrosslinking and (F) quantitative cell viability of
the printed dermal constructs 1 h after printing. Scale bars: 50 μm.

152 International Journal of Bioprinting (2022)–Volume 8, Issue 4