Page 178 - IJB-9-1

International Journal of Bioprinting Lumen-forming colorectal cancer organoids

structure of 0.5 cm in height maintained its original shape Data curation: Jiayi Xu, Fatimah Al-Jalih, Manola Moretti
even after more than 7 days post printing. We also found Formal analysis: Fatimah Al-Jalih
that the cells had a comparable proliferation rate to that in Methodology: Rosario Pérez-Pedroza, Manola Moretti,
Matrigel when low concentration was used. This provides Charlotte A. E. Hauser
evidence that this modified peptide hydrogel can be a Resources: Charlotte A. E. Hauser
promising bioink based on synthetic material that provides Supervision: Rosario Pérez-Pedroza, Manola Moretti,
a suitable, bioprintable, and biocompatible scaffold, which Charlotte A. E. Hauser
mimics the ECM from functional tissues, and in which cells Validation: Fatimah Al-Jalih
can grow and proliferate. Therefore, this hybrid peptide can Writing – original draft: Fatimah Al-Jalih
be a potential cost-effective, ECM-mimicking bioink that Writing – review & editing: Rosario Pérez-Pedroza, Manola
maintains the integrity of cells in the printed constructs. Moretti, Charlotte A. E. Hauser

5. Conclusion References

This study provides valuable insights on the effect and 1. Tuveson D, Clevers H, 2019, Cancer modeling meets human
performance of IKVAV motif when used in combination organoid technology. Science, 364(6444): 952–955.
with the ultrashort peptide IIFK as a bioink with CRC
cells. Three-dimensional constructs that positively affect https://doi.org/10.1126/science.aaw6985
cell viability can be effectively printed by utilizing the IIFK 2. Sharick JT, Jeffery JJ, Karim MR, et al., 2019, Cellular
peptide that has been functionalized with IKVAV. Confocal metabolic heterogeneity in vivo is recapitulated in tumor
microscopy showed signs of improved lumen formation organoids. Neoplasia, 21(6): 615–626.
when cells were cultured in the 3D environment of IIFK:IDP https://doi.org/10.1016/j.neo.2019.04.004
hydrogel scaffold. Importantly, it provides evidence that the 3. Luo C, Lancaster MA, Castanon R, et al., 2016, Cerebral
use of IKVAV in combination with IIFK peptide successfully organoids recapitulate epigenomic signatures of the human
delivered signals to direct lumen formation, which increased fetal brain. Cell Rep, 17(12): 3369–3384.
the lumen area in the organoid formation of CRC cells as
compared to other peptides. Moreover, the SAP constructs https://doi.org/10.1016/j.celrep.2016.12.001
were successfully applied to 3D bioprinting, where they 4. van de Wetering M, Francies HE, Francis JM, et al., 2015,
provided a suitable milieu for the growth of colorectal cancer Prospective derivation of a living organoid biobank of
colonies. This seminal study paves the way to the use of colorectal cancer patients. Cell, 161(4): 933–945.
biofunctional peptides based on SAPs hydrogels as bioinks https://doi.org/10.1016/j.cell.2015.03.053
to provide active ECM for 3D bioprinting of CRC organoids.
5. Fair KL, Colquhoun J, Hannan NRF, 2018, Intestinal
organoids for modelling intestinal development and disease.
Acknowledgments Philos Trans R Soc Lond B Biol Sci, 373(1750): 20170217.
The authors would like to acknowledge Hepi H. Susapto https://doi.org/10.1098/rstb.2017.0217
for supporting the NMR spectra analysis and general 6. Clevers H, Tuveson DA, 2019, Organoid models for cancer
advice; Hamed I. Albalawi and Ali Aldhouki for training research. Annu Rev Cancer Biol, 3(3): 223–234.
on 3D printing and laboratory support; Hamed I. Albalawi
for designing graphical abstract; and KAUST’s Bioscience https://doi.org/10.1146/annurev-cancerbio-030518-055702
and Imaging Core Labs for supporting the biological 7. Sato T, Clevers H, 2015, SnapShot: Growing organoids from
characterization and microscopy analyses. stem cells. Cell, 161(7): 1700–1700 e1.
https://doi.org/10.1016/j.cell.2015.06.028
Funding
8. Sato T, Stange DE, Ferrante M, et al., 2011, Long-term
This work was supported by KAUST baseline funding and expansion of epithelial organoids from human colon,
CBRC funding. adenoma, adenocarcinoma, and Barrett’s epithelium.
Gastroenterology, 141(5): 1762–1772.
Conflict of interest https://doi.org/10.1053/j.gastro.2011.07.050
The authors declare no conflicts of interest. 9. Bernal PN, Bouwmeester M, Madrid-Wolff J, et al., 2022,
Volumetric bioprinting of organoids and optically tuned
Author contributions hydrogels to build liver-like metabolic biofactories. Adv
Mater, 34(15): e2110054.
Conceptualization: Rosario Pérez-Pedroza, Charlotte A. E.
Hauser https://doi.org/10.1002/adma.202110054

Volume 9 Issue 1 (2023)olume 9 Issue 1 (2023)
V 170 https://doi.org/10.18063/ijb.v9i1.633