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International Journal of Bioprinting Lumen-forming colorectal cancer organoids

Figure 2. Physicochemical characterization of IIFK, IDP, and IIFK:IDP (1:1) mixture. (A) Chemical structure of the parent peptide IIFK and biofunctional
peptide IDP. (B) Inverted vial test results for CGC and gelation time. IDP generates a translucent gel that gelates with a higher CGC compared to the parent
peptide IIFK. The IIFK:IDP (1:1) mixture shows a higher CGC and higher gelation time for all 3. (C) SEM micrographs of IIFK, IDP, and IIFK:IDP. (D)
AFM height topography images for the gelated peptides at 8 mM. (E) CD spectroscopy of the IIFK:IDP mixture at different concentrations. (F) Stiffness of
the gelated peptides at varying concentrations measured by rheological analyses.

Table 1. Characteristic lengths of the fibers formed by the three peptides IIFK, IDP, and mixture of both (Mix) according to AFM
topography

Peptide Height (nm) Periodicity (nm)
IIFK 7.23 ± 0.76 55.58 ± 1.93
IDP 4.38 ± 1.05 31.40 ± 9.76
Mix 5.92 ± 0.66 56.31 ± 9.08

Volume 9 Issue 1 (2023)olume 9 Issue 1 (2023) 165 https://doi.org/10.18063/ijb.v9i1.633
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