Page 168 - IJB-9-1

International Journal of Bioprinting

RESEARCH ARTICLE
Fabrication of lumen-forming colorectal cancer

organoids using a newly designed laminin-
derived bioink

Rosario Pérez-Pedroza 1,2† , Fatimah Al-Jalih 1,2† , Jiayi Xu , Manola Moretti ,
1,2
1,2
Giuseppina R. Briola , Charlotte A. E. Hauser *
1
1,2
1 Laboratory for Nanomedicine, BESE, King Abdullah University of Science and Technology
(KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
2 Computational Bioscience Research Center, King Abdullah University of Science and Technology
(KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
(This article belongs to the Special Issue: Related to 3D printing technology and materials)

Abstract
Three-dimensional (3D) bioprinting systems, which are the prominent tools for
biofabrication, should evolve around the cutting-edge technologies of tissue en-
gineering. This is the case with organoid technology, which requires a plethora of
new materials to evolve, including extracellular matrices with specific mechanical
and biochemical properties. For a bioprinting system to facilitate organoid growth, it
must be able to recreate an organ-like environment within the 3D construct. In this
† These authors contributed equally study, a well-established, self-assembling peptide system was employed to gener-
to this work. ate a laminin-like bioink to provide signals of cell adhesion and lumen formation in
*Corresponding author: cancer stem cells. One bioink formulation led to the formation of lumen with outper-
Charlotte A. E. Hauser forming characteristics, which showed good stability of the printed construct.
(charlotte.hauser@kaust.edu.sa)
Citation: Pérez-Pedroza R,
Al-Jalih F, Xu J, et al., 2023, Keywords: Laminin; Biofunctional bioink; Self-assembling peptide; Organoid; IKVAV
Fabrication of lumen-forming
colorectal cancer organoids using
a newly designed laminin-derived
bioink. Int J Bioprint, 9(1): 633.
https://doi.org/10.18063/ijb.v9i1.633 1. Introduction
Received: July 21, 2022 Three-dimensional (3D) bioprinting is widely recognized as one of the most
Accepted: August 05, 2022 prominent enabling technologies for tissue biofabrication. In view of the extensive
Published Online: November 4,
2022 use and versatility of 3D bioprinting, it is important that these systems evolve around
the cutting-edge technologies of tissue engineering. This is the case with organoid
Copyright: © 2022 Author(s).
This is an Open Access article technology. Organoids are currently one of the most studied in vitro systems for tissue
distributed under the terms of the engineering approaches. These self-organizing cellular structures can recapitulate
Creative Commons Attribution the diversity of cell lineages and functionalization of human tissues, beginning
License, permitting distribution [1]
and reproduction in any medium, from single pluripotent cells . Organoids are suitable for studying and investigating
[2]
provided the original work is organ behavior and function, as they are able to recapitulate cellular metabolism ,
properly cited. epigenomic signatures , gene expression , and even developmental programming
[3]
[4]
[5]
Publisher’s Note: Whioce from the original tissue. At present, particularly in this fascinating period of time with
Publishing remains neutral with personalized treatment, big data management, and fast-rising innovative technologies,
regard to jurisdictional claims in
published maps and institutional there is a high demand for new methods that can combine the benefits of organoid
[6]
affiliations. models with the precision of 3D bioprinting .

Volume 9 Issue 1 (2023) 160 https://doi.org/10.18063/ijb.v9i1.633