Page 22 - IJB-9-1

International Journal of Bioprinting

RESEARCH ARTICLE
3D printing biocompatible materials with Multi

Jet Fusion for bioreactor applications

1
1†
Balasankar Meera Priyadarshini , Wai Kay Kok , Vishwesh Dikshit ,
1†
4
Shilun Feng , King Ho Holden Li *, Yi Zhang 1
2,3
1 HP-NTU Digital Manufacturing Corporate Lab, Nanyang Technological University, 639798,
Singapore
2 State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information
Technology, Chinese Academy of Sciences, Shanghai, 200050, China
3 School of Electrical and Electronic Engineering, Nanyang Technological University, 639798,
Singapore
4 School of Mechanical and Aerospace Engineering, Nanyang Technological University, 639798,
Singapore

Abstract

In the evolving three-dimensional (3D) printing technology, the involvement of
different materials in any new 3D printing process necessitates a thorough evaluation
of the product’s biocompatibility for biomedical application. Here, we examined
the ability of Multi Jet Fusion (MJF)-printed PA-12 to support cell proliferation and
osteogenesis. Our results show that leachate from MJF-printed PA-12 does not inhibit
† These authors contribute equally to the growth of L929 fibroblast and MC3T3e1 osteoblast. The substrate supports the
this work. attachment and proliferation of both cell types, though not at a level comparable to
*Corresponding author: conventional polystyrene culture plate. Neither plasma treatment, poly-D-lysine, nor
King Ho Holden Li collagen coatings narrowed the gap substantially, suggesting the possible influence of
(holdenli@ntu.edu.sg)
other limiting factors. The substrate can also support MC3T3e1 osteogenesis. However,
Citation: Priyadarshini BM, MJF-printed PA-12 exhibits varying ability in supporting the proliferation of different
Kok WK, Dikshit W, et al., 2023, cell types, especially in subsequent passages. While L929’s proliferation is comparable
3D printing biocompatible materials
with Multi Jet Fusion for bioreactor from passage-to-passage, MC3T3e1’s growth ability is noticeably compromised.
applications. Int J Bioprint, Interestingly, our results show that L929 subcultured back to polystyrene plate retains
9(1): 623. the ability to grow as robustly as those on the conventional plate, suggesting that
https://doi.org/10.18063/ijb.v9i1.623
MJF-printed PA-12 does not permanently impair cell proliferation. In addition, we
Received: April 13, 2022 have shown the successful culture of bacterial Escherichia coli on MJF-printed PA-12.
Accepted: July 01, 2022
Published Online: October 22, Together, our study demonstrated the potential of MJF-printed PA-12 for biological
2022 applications.
Copyright: © 2022 Author(s). This
is an Open Access article
distributed under the terms of the Keywords: 3D printing; PA-12; Nylon; Multi Jet fusion; Cell culture; Bacteria fouling;
Creative Commons Attribution Protein fouling; Bioreactors
License, permitting distribution, and
reproduction in any medium,
provided the original work is
properly cited.
1. Introduction
Publisher’s Note: Whioce
Publishing remains neutral with Three-dimensional (3D) printing has presented new opportunities for advanced
regard to jurisdictional claims in
published maps and institutional manufacturing of a wide spectrum of materials such as metals, ceramics, composites,
affiliations. smart materials, as well as combinations of these materials, due to its innate ability of

Volume 9 Issue 1 (2023) 14 https://doi.org/10.18063/ijb.v9i1.623