Page 18 - IJB-9-1

International Journal of Bioprinting Biocompatible materials and Multi Jet Fusion

Finally, bioinks based on alginate, gelatine, and Author contributions
nanocellulose have also been extensively investigated for Conceptualization: Julia Anna Semba, Adam Aron Mieloch,
bone tissue engineering. Besides enhancing printability, Jakub Dalibor Rybka
cellulose also increases the expression of the osteogenic Investigation: Julia Anna Semba, Ewa Tomaszewska, Piotr
marker gene [22,68] . Dutta et al. observed notable gene Cywoniuk
expression changes; however, the mesenchymal stem cells Methodology: Julia Anna Semba, Adam Aron Mieloch
were seeded on the construct composed of 3% alginate, Supervision: Adam Aron Mieloch, Jakub Dalibor Rybka
4% gelatin, and 1% cellulose nanocrystals rather than Writing – original draft: Julia Anna Semba
being encapsulated inside the bioink . Nevertheless, Writing – review & editing: Adam Aron Mieloch, Jakub
[68]
only osteogenic-specific genes were studied. Finally, a Dalibor Rybka
comparable bioink formulation of 2.0% alginate, 3.3%
gelatin, and 0.93% diethylaminoethyl cellulose was used
for skin bioprinting, yielding promising results [69,70] . These References
studies suggest that the proposed bioink could be used for 1. Pereira H, Varatojo R, Sevivas N, et al., 2016, Physiopathology
other 3D bioprinting applications.
of the meniscal lesions, in: Surgery of the Meniscus, Springer
Berlin Heidelberg, 47–61.
5. Conclusion
https://doi:10.1007/978-3-662-49188-1_5
This study presents the formulation and evaluation of 2. Doral MN, Bilge O, Huri G, et al., 2018, Modern treatment
a bioink dedicated to extrusion-based 3D bioprinting of meniscal tears. EFORT Open Rev, 3:260–268.
of meniscal tissue. The rheological analysis included
the amplitude sweep test, temperature sweep test, and https://doi.org/10.1302/2058-5241.3.170067
rotation. The selected bioink was used for bioprinting with 3. Beaufils P, Becker R, Kopf S, et al., 2017, The knee meniscus:
normal human knee articular chondrocytes. Subsequently, Management of traumatic tears and degenerative lesions.
the encapsulated cell viability and the gene expression of EFORT Open Rev, 2:195–203.
chondrogenic markers were investigated. In the course https://doi.org/10.1302/2058-5241.2.160056
of rheological and biological analyses, we established an 4. Vaishya R, Patralekh MK, Vaish A, et al., 2018, Publication
optimal bioink composition and proved that the bioink trends and knowledge mapping in 3D printing in
is printable, stable in cell culture, biocompatible, and able orthopaedics. J Clin Orthop Trauma, 9:194–201.
to maintain the native phenotype of chondrocytes. We https://doi.org/10.1016/j.jcot.2018.07.006
intend to investigate the chondrogenic potential of bioink
with human adipose-derived mesenchymal stem cells. In 5. Semba JA, Mieloch AA, Rybka JD, 2020, Introduction to
our ongoing research, the formulated bioink is used as the state-of-the-art 3D bioprinting methods, design, and
applications in orthopedics. Bioprinting, 18:e00070.
a basis to promote the chondrogenesis of encapsulated
cells through supplementation with hyaluronic acid, https://doi.org/10.1016/j.bprint.2019.e00070
carbon nanotubes, or collagen and alterations in alginate 6. Agarwal S, Saha S, Balla VK, et al., 2020, Current
crosslinking. developments in 3D bioprinting for tissue and organ
regeneration–A review. Front Mech Eng, 6:589171.
Acknowledgments https://doi.org/10.3389/fmech.2020.589171
The authors would like to thank Prof. Filip Górski and 7. Luo W, Song Z, Wang Z, et al., 2020, Printability
Dr. Anna Maria Mleczko. Open access was cofounded by optimization of gelatin-alginate bioinks by cellulose
nanofiber modification for potential meniscus bioprinting.
Excellence Initiative—Research University program, Call J Nanomater.
No. 040 “Open Access.”
https://doi.org/10.1155/2020/3863428
Funding 8. Stanco D, Urbán P, Tirendi S, et al., 2020, 3D bioprinting for
orthopaedic applications: Current advances, challenges and
This work was supported by the National Center for regulatory considerations. Bioprinting, 20:e00103.
Research and Development TECHMATSTRATEG- https://doi.org/10.1016/j.bprint.2020.e00103
III/0027/2019-00 grant.
9. Ma X, Liu J, Zhu W, et al., 2018, 3D bioprinting of functional
tissue models for personalized drug screening and in vitro
Conflict of interest disease modeling. Adv Drug Deliv Rev, 132:235–251.
The authors declare no conflicts of interest. https://doi.org/10.1016/j.addr.2018.06.011

Volume 9 Issue 1 (2023) 10 https://doi.org/10.18063/ijb.v9i1.621