Page 193 - IJB-9-3

International Journal of Bioprinting

RESEARCH ARTICLE
3D-printed vascularized biofunctional scaffold

for bone regeneration

Bojun Cao 1,2,3† , Jieming Lin , Jia Tan 1,2,3† , Jiaxin Li , Zhaoyang Ran 1,2,3 ,
5
4†
Liang Deng 1,2,3 , Yongqiang Hao 1,2,3 *
1 Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai
Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011,
China
2 Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s
Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
3 Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized
Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine,
Shanghai, 200011, China
4 Department of Orthopaedic Surgery, Renji Hospital, South Campus, Shanghai Jiao Tong University
School of Medicine, Shanghai 201112, China
5 Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin,
China
(This article belongs to the Special Issue: Novel Methods, Processes, and Materials of Bioprinting)

Abstract

3D-printed biofunctional scaffolds have promising applications in bone tissue
regeneration. However, the development of bioinks with rapid internal vascularization
capabilities and relatively sustained osteoinductive bioactivity is the primary technical
† These authors contributed equally challenge. In this work, we added rat platelet-rich plasma (PRP) to a methacrylated
to this work. gelatin (GelMA)/methacrylated alginate (AlgMA) system, which was further modified
*Corresponding author: by a nanoclay, laponite (Lap). We found that Lap was effective in retarding the release of
Yongqiang Hao multiple growth factors from the PRP-GelMA/AlgMA (PRP-GA) hydrogel and sustained
(hyq_9hospital@hotmail.com) the release for up to 2 weeks. Our in vitro studies showed that the PRP-GA@Lap hydrogel
Citation: Cao B, Lin J, Tan J, et al., significantly promoted the proliferation, migration, and osteogenic differentiation of
2023, 3D-printed vascularized rat bone marrow mesenchymal stem cells, accelerated the formation of endothelial
biofunctional scaffold for bone
regeneration. Int J Bioprint, cell vascular patterns, and promoted macrophage M2 polarization. Furthermore, we
9(3): 702. printed hydrogel bioink with polycaprolactone (PCL) layer-by-layer to form active bone
https://doi.org/10.18063/ijb.702 repair scaffolds and implanted them in subcutaneous and femoral condyle defects
Received: December 3, 2022 in rats. In vivo experiments showed that the PRP-GA@Lap/PCL scaffolds significantly
Accepted: January 1, 2023 promoted vascular inward growth and enhanced bone regeneration at the defect site.
Published Online: March 8, 2023
This work suggests that PRP-based 3D-bioprinted vascularized scaffolds will have great
Copyright: © 2023 Author(s). potential for clinical translation in the treatment of bone defects.
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution Keywords: 3D bioprinting; Vascularization; Platelet-rich plasma; Bone regeneration
License, permitting distribution
and reproduction in any medium,
provided the original work is
properly cited.
1. Introduction
Publisher’s Note: Whioce
Publishing remains neutral with The repair and functional reconstruction of bone defects is a major issue in the field of
regard to jurisdictional claims in orthopedics that needs to be addressed . The “gold standard” in the treatment of bone
[1]
published maps and institutional
affiliations. defects is autologous bone grafting; nevertheless, their clinical application is hindered

Volume 9 Issue 3 (2023) 185 https://doi.org/10.18063/ijb.702