Page 37 - IJB-9-4

International Journal of Bioprinting

RESEARCH ARTICLE
3D-bioprinted human lipoaspirate-derived cell-

laden skin constructs for healing of full-thickness
skin defects

Dequan Zhang , Qiang Fu , Huijuan Fu , Jinshi Zeng , Litao Jia ,
1,2
1
3
3
1
Minliang Chen *
1
1 Senior Department of Burns and Plastic Surgery, the Fourth Medical Center of PLA General
Hospital, Beijing, 100048, China
2 Central Medical Branch of PLA General Hospital, Beijing, 100120, China
3 Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking
Union Medical College, Beijing, 100144, China

Abstract

Three-dimensional (3D)-printed bioactive scaffolds that can be produced rapidly
could offer an individualized approach for treating full-thickness skin defects.
Decellularized extracellular matrix (dECM) and mesenchymal stem cells have been
proven to support wound healing. Adipose tissues obtained by liposuction are rich
in adipose-derived dECM (adECM) and adipose-derived stem cells (ADSCs) and
thus represent a natural source of bioactive materials for 3D bioprinting. Herein,
ADSC-laden 3D-printed bioactive scaffolds consisting of gelatin methacryloyl
(GelMA), hyaluronic acid methacryloyl (HAMA), and adECM were fabricated with
dual properties of photocrosslinking in vitro and thermosensitive crosslinking
*Corresponding author: in vivo. adECM was prepared by decellularization of human lipoaspirate and mixed
Minliang Chen
(Chenml@sohu.com) as a bioactive material with GelMA and HAMA to form a bioink. Compared with
the GelMA–HAMA bioink, the adECM–GelMA–HAMA bioink had better wettability,
Citation: Zhang D, Fu Q, Fu H, degradability, and cytocompatibility. Full-thickness skin defect healing in a nude
et al., 2023, 3D-bioprinted human
lipoaspirate-derived cell-laden mouse model showed that ADSC-laden adECM–GelMA–HAMA scaffolds accelerated
skin constructs for healing of full- wound healing by promoting faster neovascularization, collagen secretion, and
thickness skin defects. Int J Bioprint, remodeling. ADSCs and adECM collectively conferred bioactivity on the prepared
9(4): 718.
https://doi.org/10.18063/ijb.718 bioink. This study represents a novel approach to enhancing the biological activity
of 3D-bioprinted skin substitutes by adding adECM and ADSCs derived from human
Received: December 20, 2022
Accepted: February 07, 2023 lipoaspirate and may provide a promising therapeutic option for full-thickness skin
Published Online: March 23, 2023 defects.
Copyright: © 2023 Author(s).
This is an Open Access article
distributed under the terms of the Keywords: Lipoaspirate; Adipose-derived stem cells; Adipose-derived decellularized
Creative Commons Attribution extracellular matrix; 3D bioprinting; Full-thickness skin defect; Wound healing
License, permitting distribution
and reproduction in any medium,
provided the original work is
properly cited.
1. Introduction
Publisher’s Note: Whioce
Publishing remains neutral with In China, the treatment and nursing of cutaneous wounds create a heavy burden on
regard to jurisdictional claims in [1]
published maps and institutional individuals, families, and society . According to studies conducted in the USA, medical
affiliations. expenses incurred by repairing various wounds, particularly surgical wounds and diabetic

Volume 9 Issue 4 (2023) 29 https://doi.org/10.18063/ijb.718