Page 133 - IJB-9-5

International Journal of Bioprinting DLP-printed scaffold for bone regeneration

Combined with the mineralization and osteogenesis of Resources: Licheng Zhang, Xing Wang
cartilage at week 4 and week 8, the adsorption of iron ions Validation: Zhongyang Liu, Junyao Cheng, Xiao Liu
and the abundant expression of HIF-1α resulted in a good Writing – original draft: Jianpeng Gao, Hufei Wang
balance between cartilage and bone due to the growth of Writing – review & editing: Jianheng Liu, Xing Wang,
blood vessels into the bone (Figures 8 and 9). Thus, the Licheng Zhang
GelMA/3%PMAA hydrogel well induced the process of
ECO and promoted the regeneration of bone. Ethics approval and consent to participate

In this study, GelMA/3%PMAA scaffolds with porous The Institutional Animal Care and Use Committee of the
structure were prepared using DLP printing technology, Chinese PLA General Hospital approved the design of this
which could further promote chondrogenic differentiation study (2022-x18-51).
of BMSCs and vascular regeneration by chelating iron ions
to promote the expression of HIF-1α without the help of Consent for publication
exogenous factors. GelMA/3%PMAA scaffolds could Not applicable.
induce ECO through their own properties to regulate
bone regeneration, providing a new approach for clinical Availability of data
treatment of bone defects, especially non-weight-bearing
bone defects manifesting weak angiogenesis. The datasets used and analyzed during the current study
are available from the corresponding author on reasonable
5. Conclusion request.
In conclusion, a biocompatible GelMA/PMAA scaffold References
was successfully developed to recapitulate and accelerate
in situ ECO-based bone regeneration through increasing 1. Gillman CE, Jayasuriya AC, 2021, FDA-approved bone
HIF-1α by adsorption of iron ions while ensuring grafts and bone graft substitute devices in bone regeneration.
structural advantages to induce cartilage differentiation Mater Sci Eng C Mater Biol Appl, 130: 112466.
and vascular regeneration. The ability to realize ECO https://doi.org/10.1016/j.msec.2021.112466
through the properties of materials via biomaterial-based
iron ion chelation provides a promising strategy for clinical 2. Bose S, Sarkar N, 2020, Natural medicinal compounds in
bone tissue engineering. Trends Biotechnol, 38(4): 404–417.
treatment of bone defects.
https://doi.org/10.1016/j.tibtech.2019.11.005
Acknowledgments 3. Ghimire S, Miramini S, Edwards G, et al., 2021, The
We thank Institute of Orthopedics, PLA General Hospital, investigation of bone fracture healing under intramembranous
and endochondral ossification. Bone Rep, 14: 100740.
for providing the experimental equipment.
https://doi.org/10.1016/j.bonr.2020.100740
Funding 4. Galea GL, Zein MR, Allen S, et al., 2021, Making and
This work was supported by the Key Program of the shaping endochondral and intramembranous bosnes. Dev
Dyn, 250(3): 414–449.
National Natural Science Foundation of China (grant
number 21935011), the Beijing Municipal Natural Science https://doi.org/10.1002/dvdy.278
Foundation (grant number L202033), and the Military 5. Chan WCW, Tan Z, To MKT, et al., 2021, Regulation and
Medical Science and Technology Youth Training Program role of transcription factors in osteogenesis. I J Mol Sci,
(grant number 19QNP052). 22(11): 5445.
https://doi.org/10.3390/ijms22115445
Conflict of interest 6. Weng Y, Wang H, Wu D, et al., 2022, A novel lineage of
The authors declare no conflict of interest. osteoprogenitor cells with dual epithelial and mesenchymal
properties govern maxillofacial bone homeostasis and
Author contributions regeneration after MSFL. Cell Res, 32(9): 814–830.
https://doi.org/10.1038/s41422-022-00687-x
Conceptualization: Jianpeng Gao, Hufei Wang
Formal analysis: Jianheng Liu, Ming Li 7. He J, Yan J, Wang J, et al., 2021, Dissecting human embryonic
Funding acquisition: Xing Wang, Jianheng Liu skeletal stem cell ontogeny by single-cell transcriptomic and
Investigation: Jianpeng Gao, Hufei Wang, Jianheng Liu functional analyses. Cell Res, 31(7): 742–757.
Methodology: Licheng Zhang, Xing Wang https://doi.org/10.1038/s41422-021-00467-z

Volume 9 Issue 5 (2023) 125 https://doi.org/10.18063/ijb.754