Page 249 - IJB-9-5

International Journal of Bioprinting

Scheme 1. Schematic presentation for methacryloylation of Antheraea pernyi silk fibroin with methacrylic anhydride (MA) (ASF-MA) and bioprinting of
cells with ASF-MA by digital light processing (DLP) 3D printer. (A) Preparation process of ASF-MA hydrogels. (B) The diagram of continuous DLP 3D
printing and 3D-printed structure. Abbreviations: DMD, digital micromirror device; LED, light-emitting diode; UV, ultraviolet.

with 1 L of 0.05 M Na CO solution at 100°C for 15 min 4°C for 3 days, and freeze-dried to obtain about 20 g of
3
2
for three times. After the sericin was removed, the ASF regenerated Antheraea pernyi silk fibroins, which were soft
was washed several times with distilled water and wrung light-brown solid.
dry. Until there was no turbid residue in the water, the ASF
were dried at room temperature. 2.2.3. Preparation of ASF-MA hydrogels
To ensure the stability of the methacryloylated ASF, 1 g
2.2.2. Regeneration of Antheraea pernyi silk fibroins of the regenerated ASF was dissolved in 10 mL of the
Five hundred grams of Ca(NO ) •4H O were placed in a prepared 0.25 M carbonate buffer (CB) solution and stirred
3 2
2
500-mL round-bottomed flask, heated and melted at 105°C in an ice bath. Then, 10, 25, 50, 100, and 150 μL of MA
in an oil bath, and slowly added with 25 g of Antheraea were slowly added and reacted for 6 h, denoted as ASF-
pernyi silk fibroins. The solution was dark brown and MA , ASF-MA 2.5% , ASF-MA , ASF-MA 10% , and ASF-
5%
1%
was stirred continuously to dissolve Antheraea pernyi MA 15% , respectively. The prepared solution was filtered and
silk fibroins. After 4 h, the solution was dialyzed against dialyzed at 4°C for 7 days. ASF-MA solutions were frozen
distilled water using 3500-kDa cutoff dialysis bags at at -80°C and freeze-dried.

Volume 9 Issue 5 (2023) 241 https://doi.org/10.18063/ijb.760