Page 257 - IJB-9-5

International Journal of Bioprinting

Figure 4. (A) The system composition of the DLP 3D printer. (B) The different 3D-printed structures. (C) The 3D-bioprinted structures of the “peace dove”
and the “Yin-Yang Tai Chi” diagram. The red fluorescence represented CT26-RFP RED cells, and the green fluorescence represented OP9-GFP GREEN
cells.
computer-aided design (CAD) images. The principle of as a result of the light. The item rises gradually for the
the continuous DLP 3D printing technology is illustrated resin to flow below and stay in touch with the bottom.
in Figure 4A. Since ASF-MA and GelMA materials had no The printing process is continuous . We performed
[40]
obvious temperature changes during the curing process, DLP 3D printing with the ASF-MA bioink, which could
continuous 3D printing could be directly performed produce complex structures such as hollow structures and
without lifting and cooling. By improving the system “CHINA” structures with high precision (Figure 4B). We
composition of the DLP 3D printer, the liquid pool that printed fluorescent cells mixed with ASF-MA bioinks.
should be filled with printing ink was removed, and the This method could visually reflect the survival of cells
printing ink was brought into between the printing plane inside the hydrogel. We printed the complex structure of
and the sample stage through the interfacial tension of the the “peace dove ” and the “Yin-Yang Tai Chi” diagram
liquid. Thus, the waste of printing ink was reduced [37-39] . (Figure 4C). The CT26-RFP RED cells at the edge of the
Through the glass, a beam of ultraviolet light illuminates “peace dove” pattern had gradually grown from the inside
the exact cross-section of the item. The resin solidifies of the hydrogel to the outside by the third day. Hydrogels

Volume 9 Issue 5 (2023) 249 https://doi.org/10.18063/ijb.760