Page 88 - IJB-9-5

International Journal of Bioprinting Biocompatible BSA-GMA and TPP of 3D hydrogels with free radical type I photoinitiator

Figure 8. 3D images of confocal fluorescence microscopy of the stained chondrocytes on five hydrogel scaffolds (R D , R D , R D , R D , and R D ).
52
40
40
15
35
30
52
20
52
40
(A) 3D images of confocal fluorescence microscopy of living/dead stained chondrocytes on five hydrogel scaffolds. (B) Overlay of the 3D optical section of
living cells stained by Hoechst, PI, and Mito-Tracker Deep Red on five hydrogel scaffolds.
for the 3D culture of living cells and could be used as a The authors also thank Dr. Ye Tian and Prof. Xian-
cellular scaffold for subsequent cartilage repair studies. Wei Meng for the discussion about Zeta potential
characterization.
4. Conclusion
Funding
In summary, we demonstrate that GMA-modified BSA
(BSA-GMA) can enable the TPP of BSA-GMA in the This work was financially supported by the International
aqueous phase with the radical type I photoinitiator of Partnership Program of Chinese Academy of Sciences
LAP. The as-prepared BSA-GMA reduces the consumption (GJHZ2021130), National Natural Science Foundation
of a large number of amino acids during TPP compared to of China (NSFC, Grant Nos. 61975213, 51901234, and
pure BSA. The TPP capability can be regulated by adjusting 61475164), the National Key R&D Program of China
the degree of methacrylation and concentration. The 3D (Grant Nos. 2017YFB1104300 and 2016YFA0200500), and
BSA-GMA hydrogel structures with fine configuration, Cooperative R&D Projects between FFG of Austria and
autofluorescence, pH response, and biocompatibility CAS of China (GJHZ1720).
have been fabricated by femtosecond laser direct writing
technique. The chondrocytes attach and grow well on the Conflict of interest
scaffold since the surface of BSA is negatively charged The authors declare no conflict of interest.
and similar to the cartilage growth environment in a
physiological environment (pH 7.4). Benefiting from the Author contributions
unique optical property and biocompatibility, BSA-GMA
hydrogels have good prospects for various biomedical Conceptualization: Teng Li, Mei-Ling Zheng
applications. Subsequent research will be directed toward Data curation: Jie Liu, Mei-Ling Zheng
the application of BSA-GMA hydrogels for drug release Formal analysis: Teng Li, Min Guo, Fan-Chun Bin, Atsushi
and cartilage repair. Nakayama
Investigation: Teng Li, Jie Liu, Wei-Cai Zhang, Jian-Yu
Acknowledgments Wang, Mei-Ling Zheng
Methodology: Teng Li, Katsumasa Fujita, Mei-Ling Zheng
The authors thank the financial support of the International Project administration: Katsumasa Fujita, Mei-Ling Zheng
Partnership Program of Chinese Academy of Sciences Software: Feng Jin, Xian-Zi Dong
(GJHZ2021130), National Natural Science Foundation Supervision: Mei-Ling Zheng
of China (NSFC, Grant Nos. 61975213, 51901234, and Validation: Feng Jin, Xian-Zi Dong
61475164), the National Key R&D Program of China Visualization: Jie Liu
(Grant Nos. 2017YFB1104300 and 2016YFA0200500), and Writing – original draft: Teng Li
Cooperative R&D Projects between FFG of Austria and Writing – review & editing: Mei-Ling Zheng
CAS of China (GJHZ1720).

Volume 9 Issue 5 (2023) 80 https://doi.org/10.18063/ijb.752