Page 83 - IJB-9-6

International

Journal of Bioprinting

REVIEW ARTICLE
Organoid bioprinting strategy and application in

biomedicine: A review

Chen He , Jiasheng Yan , Yusheng Fu , Jiuchuan Guo , Yuxing Shi , and
2
1
1
1
1
Jinhong Guo *
1,2
1 School of Information and Communication Engineering, University of Electronic Science and
Technology of China, Chengdu, China
2 School of Sensing Science and Engineering, Shanghai Jiao Tong University, Shanghai, China
(This article belongs to the Special Issue: Bioprinting Tissues for Disease Modeling, Drug Development and
Drug Testing)
Abstract

Organoids are three-dimensional cell structures cultured in vitro. They are self-
organizing and can mimic real organs in structure and function. Bioprinting
technology breaks through some limitations of organoid manufacturing, making it
more widely used in drug screening, regenerative medicine, and other fields. In this
review, we first introduce bioinks and bioprinting methods for stem cell and organoid
bioprinting, then summarize several vascularization strategies for bioprinting
organoids, and present applications in biomedicine. In the future, the development
of microfluidic technology and four-dimensional bioprinting technology may be
conducive to forming better bioprinted organoids.

Keywords: Bioprinting; Organoid; Tissue engineering

*Corresponding author:
Jinhong Guo
(guojinhong@sjtu.edu.cn) 1. Introduction
Citation: He C, Yan J, Fu Y, et al., Organoids are defined as collections of organ-specific cells. These cells are cultured through
2023, Organoid bioprinting strategy
and application in biomedicine: A in vitro three-dimensional (3D) culture systems, self-organized through cell sorting and
review. Int J Bioprint, 9(6): 0112. spatially constrained cell lineage differentiation, and exhibit a high degree of structural
https://doi.org/10.36922/ijb.0112 and functional similarity to in vivo tissues or organs . According to different cell sources,
[1]
Received: April 3, 2023 organoids can be divided into tissue-derived organoids and pluripotent stem cell (PSC)-
Accepted: May 16, 2023 derived organoids. Tissue-derived organoids are composed of tissue-derived cells (TDCs),
Published Online: June 30, 2023 which include normal stem/progenitor cells, differentiated cells, and cancer cells. These
Copyright: © 2023 Author(s). TDCs are typically obtained from biopsy samples of tissue and organs from humans or
This is an Open Access article animals. On the other hand, PSC-derived organoids are generated from PSCs, such as
distributed under the terms of the
Creative Commons Attribution embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) [2,3] .
License, permitting distribution,
and reproduction in any medium, In 2009, Sato et al. successfully cultivated the first 3D organoid using mouse intestinal
provided the original work is stem cells (ISCs). They cultured intestinal organoids with crypt-villus by planting
properly cited. mouse intestinal adult stem cells into matrix glue in vitro, using a serum-free medium
[4]
Publisher’s Note: AccScience supplemented with three growth factors: R-spondin1, EGF, and Noggin . Since then,
Publishing remains neutral with the field of organoids has developed rapidly. At present, many types of organoids have
regard to jurisdictional claims in [5,6] [7] [8] [9] [10]
published maps and institutional been successfully constructed, including heart , kidney , liver , brain , and tumor
affiliations. organoids.

Volume 9 Issue 6 (2023) 75 https://doi.org/10.36922/ijb.0112