Page 57 - JCBP-2-3

Journal of Clinical and
Basic Psychosomatics Proteomic analysis of mind-body psychotherapy in psoriasis

A B

C D

Figure 3. Differentially expressed proteins (DEPs) identified in proteomics analysis comparing protein profiles before and after MMIP. (A) Volcano plot
shows significantly increased proteins (n = 4) and decreased proteins (n = 12) after therapy. (B) Heatmap shows significantly altered proteins after therapy
(Therapy) and before therapy (Disease). (C) KEGG pathway analysis shows three significantly altered pathways after the therapy. (D) PPI figure shows the
interaction of DEPs (Table 3 presents the full name of proteins).
Abbreviations: MMIP: Mongolian mind-body interactive psychotherapy; KEGG: Kyoto Encylopedia of Genes and Genomes; PPI: Protein-protein
interaction.

3.6. Venn diagram of proteomic analysis with psoriasis. MMIP therapy alters Fc receptor-mediated
Figure 5 shows a venn diagram of eight DEPs (Q0ZCH6, phagocytosis pathways, tight junction, and vascular
PEBP1, ACTA2, CAP1, VASP, B4DRA0, PSMD9, and smooth muscle contraction. In addition, our study showed
ELAF) that were identified in both groups ([Disease vs. that MMIP altered Fc receptor-mediated pathology in
Health] & [Therapy vs. Disease]). Among these proteins, psoriasis. Further, we validated the changes of ELAF levels
six of them significantly decreased after the MMIP, except in the serum of psoriasis patients by ELISA.
Q0ZCH6 and CAP1 (Figure 3B and Table 3). The low Ig
levels in psoriasis (nine types of Ig compared with HCs, 4. Discussion
Table 2) may be related to immune deficiency and/or This is the first study of proteomics analysis of the
enhanced ADCC activity against self-antigens through exosomes derived from plasma of psoriasis patients.
Fc receptors. Alternatively, the increase of Ig levels after The exosome contains various cargos that are involved
therapy (Q0ZCH6) also suggests the recovery of immune in cellular communications, and proteomics analysis of
status and/or the decrease of ADCC activity against self- the exosome showed that molecular profile, which was
antigens. In addition, the inflammatory markers of ELAF pathologically altered in psoriasis, could be improved by
and PEBP1 were significantly elevated in patients with MMIP. Comparisons were conducted based on disease
psoriasis and decreased after the therapy. status (between psoriasis patients and HCs) and MMIP

In summary, the results showed that the involvement of treatment status (between pre- and post-MMIP among
ribosome and apelin signaling is pathologically associated psoriasis patients).

Volume 2 Issue 3 (2024) 9 doi: 10.36922/jcbp.2381