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Journal of Clinical and Translational Research 2024; 10(6): 348-356




                                       Journal of Clinical and Translational Research

                                              Journal homepage: http://www.jctres.com/en/home


        ORIGINAL ARTICLE


        Effect of high-intensity interval and endurance training with MitoQ on
        mitochondrial dynamics in rat muscle



        Soheil Aminizadeh 1  , Hamid Najafipour 2  , Yaser Masoumi-Ardakani 3  , Beydolah Shahouzehi * ,
                                                                                            4
        Mohammad Pourranjbar 5
        1 Physiology Research Center, Institute of Neuropharmacology, Department of Physiology and Pharmacology, Afzalipour School of Medicine,
        Kerman University of Medical Sciences, Kerman, Iran,  Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences,
                                                2
        Kerman University of Medical Sciences, Kerman, Iran,  Endocrinology and Metabolism Research Center, Institute of Basic and Clinical
                                                3
        Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran,  Gastroenterology and Hepatology Research Center, Institute of
                                                               4
        Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran,  Department of Physical Education, Faculty of
                                                                             5
        Medicine and Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran
        ARTICLE INFO                       ABSTRACT

        Article history:                   Background  and  Aim: Mitochondria play an important role in signaling and metabolic
        Submitted: July 22, 2024           pathways in skeletal muscle. In this study, the effects of MitoQ supplementation alone and
        Accepted: December 9, 2024         in combination with endurance training (ET) or high-intensity interval training (HIIT) were
        Published Online: December 24, 2024  investigated in relation to the process of mitochondrial quality control in the gastrocnemius
                                           muscle of male rats.
        Keywords:                          Methods: Animals were assigned into 6 groups (n = 7): Control, MitoQ, ET, ET + MitoQ, HIIT,
        MitoQ                              and HIIT + MitoQ. The gene and protein expression were quantified using real-time polymerase
        Exercise training                  chain reaction (2 -ΔΔCT ) and Western blot analysis, respectively. Statistical analysis was performed
        Fission                            using one-way analysis of variance.
        Fusion                             Results: ET significantly increased protein expression of dynamin-related protein 1 (DRP1) and
        Mitochondrial dynamic              mitofusin1 (MFN1) and gene expression of optic atrophy Type 1 (Opa1) in skeletal muscle, when
                                           compared to the control group (p < 0.001). HIIT only increased MFN1 protein expression compared
        *Corresponding author:             to the control group (p < 0.0001). MitoQ in combination with HIIT significantly increased protein
        Beydolah Shahouzehi                expression of DRP1 and MFN1 compared to MitoQ alone (p < 0.01).
        Gastroenterology and Hepatology Research   Conclusion: In sum, exercise training can affect mitochondrial dynamics by changing the factors
        Center, Institute of Basic and Clinical   involved in the fission and fusion process, and ET can improve training capacity in skeletal muscle
        Physiology Sciences, Kerman University of   by  modulating  expression  of  OPA1  and  MFN1.  While  MitoQ  supplementation  alone  did  not
        Medical Sciences, Kerman, Iran.    significantly alter the mitochondrial fission-fusion process, its combination with HIIT appeared
        Email: bshahouzehi@gmail.com       to elevate the expression of DRP1, suggesting a potential synergistic effect that warrants further
                                           investigation. Future studies should delve into the mechanisms by which MitoQ and exercise-
        © 2024 Author(s). This is an Open-Access   induced stress affect mitochondrial quality control, particularly in the context of redox modulation
        article distributed under the terms of the   and signaling pathways that govern mitochondrial plasticity.
        Creative Commons Attribution-Noncommercial
        License, permitting all non-commercial use,   Relevance for Patients: Combining MitoQ with exercise training may enhance mitochondrial
        distribution, and reproduction in any medium,   function, potentially improving muscle health in patients.
        provided the original work is properly cited.

                                           1. Introduction
                                             Regarded as dynamic organelles, mitochondria change their shape and structure in
                                           response to various metabolic stimuli. Mitochondrial structure is mainly regulated by
                                           fusion  and  fission  cycles.  These  processes  are  tightly  regulated  to  ensure  a  balance.
                                           Dysregulated  fusion  and  fission  of  mitochondria  is  an  important  mechanism  in  the
                                           development  of some diseases [1]. Mitochondrial  dysfunction results in chronic

                                               DOI: http://doi.org/10.36922/jctr.24.00044
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