Page 42 - JCTR-10-6

352 Aminizadeh et al. | Journal of Clinical and Translational Research 2024; 10(6): 348-356

A B

Figure 1. Protein expression of DRP1 in skeletal muscles. (A) DRP1 protein expression in different groups. (B) MFN1 protein expression in
different groups. *p < 0.05
Abbreviations: ET: Endurance training; HIIT: High-intensity interval training

Figure 2. Quantitation of Opa1 gene expression in skeletal muscles Figure 3. Quantitation of Fis1 gene expression in skeletal muscles
(mean ± SD). *p < 0.05 (mean ± SD)
Abbreviations: ET: Endurance training; HIIT: High-intensity interval Abbreviations: ET: Endurance training; HIIT: High-intensity interval
training training

exercise in the study of Yoo et al. [37] In their study, Axelrod and Opa1 gene. Axelrod et al. [38] also reported that aerobic
et al. [38] showed that treadmill aerobic exercise (5 days/ exercise (12 weeks) significantly decreased the expression of
week, 12 weeks) did not significantly alter the expression of proteins involved in mitochondrial fission (FIS1 and Parkin).
proteins related to the fusion of human muscles. OPA1 and Our data showed that aerobic training significantly increased the
mitofusins are related to mitochondrial fusion, which plays a expression of DRP1 protein and has no effect on the expression
pivotal role in muscle [34,39]. Furthermore, there are proteins, of FIS1. The data of Axelrod et al.’s study [38] are not in line
such as DRP1 and FIS1 which are involved in damaged with our findings, probably due to the longer duration of exercise
mitochondria elimination through mitochondrial fission [39]. in their study and its implementation on human subjects [38].
We showed that exercise, especially aerobic exercise training, It has been demonstrated that old mice exhibited increased
caused a significant increase in the expression of MFN1 protein protein expression of FIS1 and decreased level of MFN2, which

DOI: http://doi.org/10.36922/jctr.24.00044