Page 46 - JCTR-11-1

Journal of Clinical and
Translational Research Hesperidin enhances repair of γ-irradiated wounds

A B

C D

Figure 1. Effect of hesperidin treatment on contraction of excision wound in the skin of mice exposed to different doses of fractionated γ-radiation. Wound
area percentage of day 1 in (A) a dose-response manner and after exposure to (B) 10 Gy, (C) 20 Gy, and (D) 40 Gy.
Abbreviations: CMC: Carboxymethylcellulose; HPD: Hesperidin; IR: Irradiation; SIR: Sham-irradiation.

+ irradiation group (Figure 3). Hesperidin pre-treatment days 4 and 8 post-irradiation in the hesperidin + irradiation
was able to restore collagen levels nearly to baseline by day group (Figure 4).
8 post-irradiation, except for animals exposed to 40 Gy. End products of NO synthesis, specifically nitrite levels,
The increase in DNA content in treated wounds were elevated early at day 4 post-irradiation in granulation
indicates cellular hyperplasia and wound regeneration. tissue but subsequently decreased substantially in both
A rapid rise in DNA content was noted up to day 8 post- CMC and hesperidin + sham-irradiation groups. Exposure
irradiation in both the sham-irradiation groups. Exposure to various doses of fractionated γ-radiation considerably
to various doses of fractionated γ-radiation significantly decreased nitrite contents in the granulation tissues at all post-
reduced the DNA content at all post-irradiation times. irradiation times (Figure 5). The decrease in nitrite contents
However, hesperidin treatment before irradiation (10 and was significant only on day 8 (P < 0.04) after exposure to
20 Gy) resulted in a significant elevation of DNA content at 10 Gy compared with the CMC + sham-irradiation group. In

Volume 11 Issue 1 (2025) 40 doi: 10.36922/jctr.24.00049