Page 49 - JCTR-11-1

Journal of Clinical and
Translational Research Hesperidin enhances repair of γ-irradiated wounds

A B

C

Figure 7. Effect of hesperidin on glutathione peroxidase in the skin of mice exposed to different doses of fractionated γ- radiation at different post-
irradiation times. Glutathione peroxidase levels in (A) 10 Gy, (B) 20 Gy, and (C) 40 Gy. Notes: Squares indicates: CMC+Sham-irradiation; Uptriangles
indicates: Hesperidin+Sham-irradiation; Circles indicates: CMC+irradiation; Down triangles indicates: Hesperidin+irradiation.
Abbreviations: CMC: Carboxymethylcellulose; GSH: Glutathione; HPD: Hesperidin; IR: Irradiation; SIR: Sham-irradiation.

3.3.3. LPx hesperidin + irradiation groups, peaking at 3 h post-
The infliction of an excision wound caused an elevation in irradiation and declining steadily thereafter. Hesperidin pre-
LPx levels in the CMC + sham-irradiation group. LPx levels treatment provided significant protection (P < 0.05) against
fluctuated over time in both CMC + sham-irradiation and the radiation-induced LPx up to 24 h post-irradiation for
hesperidin + sham-irradiation groups, with the highest the 40 Gy group. However, the baseline LPx levels could not
elevation observed at 3 h post-irradiation, followed by be restored even by 48 h post-irradiation (Figure 8).
a gradual decline. Hesperidin treatment resulted in a 3.4. Experiment 4: Histological studies
significant (P < 0.05) decline in the baseline LPx levels
(Figure 8). Radiation exposure increased LPx levels, with Histological evaluation of wound biopsies at various post-
the highest levels observed at 40 Gy. This increase was irradiation times revealed that hesperidin treatment alone
also time-dependent in both the CMC + irradiation and did not alter wound histology, except for an increase in

Volume 11 Issue 1 (2025) 43 doi: 10.36922/jctr.24.00049