Page 48 - JCTR-11-1

Journal of Clinical and
Translational Research Hesperidin enhances repair of γ-irradiated wounds

A B

C

Figure 6. Effect of hesperidin on the glutathione contents in the skin of mice exposed to different doses of fractionated γ- radiation at different post-
irradiation times. Glutathione levels in (A) 10 Gy, (B) 20 Gy, and (C) 40 Gy. Notes: Squares indicates: CMC+Sham-irradiation; Uptriangles indicates:
Hesperidin+Sham-irradiation; Circles indicates: CMC+irradiation; Down triangles indicates: Hesperidin+irradiation.
Abbreviations: CMC: Carboxymethylcellulose; HPD: Hesperidin; IR: Irradiation; SIR: Sham-irradiation.

(Figure 6). Hesperidin treatment before irradiation caused GSHPx activity up to 48 h post-irradiation compared to the
a significant increase in the GSH contents compared to the CMC + sham-irradiation group. Exposure to different doses
concurrent CMC + irradiation group. However, even with of fractionated γ-radiation resulted in a dose-dependent
hesperidin treatment, GSH levels could not be restored to decline in GSHPx activity in both the CMC + irradiation
normal by 48 h post-irradiation in either group (Figure 6). group and hesperidin + irradiation group compared
to their respective sham-irradiation groups (Figure 7).
3.3.2. GSHPx However, the pattern of GSHPx activity fluctuations after
The activity of GSHPx in the CMC + sham-irradiation group radiation exposure was similar to that observed in the
fluctuated over time, with the highest elevation detected sham-irradiation group. The administration of hesperidin
at 1.5 h post-irradiation and a second elevation observed before exposure to various doses of γ-radiation caused a
at 24 h that remained stable up to 48 h post-irradiation drastic increment in GSHPx activity, with the highest at
(Figure 7). Hesperidin administration before sham- 1.5 h (P < 0.02) followed by 24 h (P < 0.03) post-irradiation
irradiation resulted in a significant increase (P < 0.05) in in the hesperidin + irradiation group (Figure 7).

Volume 11 Issue 1 (2025) 42 doi: 10.36922/jctr.24.00049