Page 47 - JCTR-11-1

Journal of Clinical and
Translational Research Hesperidin enhances repair of γ-irradiated wounds

Figure 2. Effect of hesperidin treatment on the progression of wound Figure 4. Effect of hesperidin on the DNA synthesis in the granulation
closure with time (mean wound healing time) in mice exposed to various tissue of regenerating deep dermal wound of mice exposed to different
doses of fractionated γ-radiation. *P < 0.05 when comparing HPD groups doses of fractionated γ- radiation at different post-irradiation times. HPD
to the CMC and SIR groups. groups are compared to CMC and SIR groups.
Abbreviations: CMC: Carboxymethylcellulose; HPD: Hesperidin; Abbreviations: CMC: Carboxymethylcellulose; HPD: Hesperidin;
SIR: Sham-irradiation. SIR: Sham-irradiation.

Figure 3. Effect of hesperidin on collagen synthesis in the granulation Figure 5. Effect of hesperidin on nitrate and nitric oxide biosynthesis
tissue of regenerating deep dermal wound of mice exposed to different in the granulation tissue of regenerating deep dermal wound of mice
doses of fractionated γ- radiation at different post-irradiation times. HPD exposed to different doses of fractionated γ- radiation at different post-
groups are compared to the CMC and SIR groups. irradiation times. HPD groups are compared to the CMC and SIR groups.
Abbreviations: CMC: Carboxymethylcellulose; HPD: Hesperidin; Abbreviations: CMC: Carboxymethylcellulose; HPD: Hesperidin;
SIR: Sham-irradiation. SIR: Sham-irradiation.

addition, a significant decline in nitrite levels was observed increased at 6 h post-irradiation in the CMC + sham-
on days 4 (P < 0.005), 8 (P < 0.0004), and 12 (P < 0.005) irradiation group, followed by a second decrement at 48 h
post-irradiation for 40 Gy compared to the CMC + sham- post-irradiation. However, treatment with hesperidin
irradiation group. Hesperidin treatment resulted in an before sham-irradiation resulted in a significant
elevation in nitrite levels that were statistically significant at (P < 0.05) elevation in GSH levels at all post-irradiation
days 4 and 8 post-irradiation for 10 Gy (Figure 5). times (Figure 6). Exposure to various doses of fractionated
γ-radiation resulted in a drastic decline in the GSH
3.3.1. GSH contents compared to the CMC + sham-irradiation

GSH content started to decline immediately after wound group. This decline was dose-dependent in both the
creation, reaching its lowest point within 3 h. It then CMC + irradiation and hesperidin + irradiation groups

Volume 11 Issue 1 (2025) 41 doi: 10.36922/jctr.24.00049