Page 87 - JCTR-11-4

Journal of Clinical and
Translational Research NADPH oxidase inhibition in a rodent stroke model

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Figure 2. Effect of NADPH oxidase inhibition on post-MCAO infarct development, at acute (48 h) and chronic (11 days) post-MCAO time points,
alongside post-MCAO survival probability. In addition, the effect of VAS2870 treatment following MCAO on well-being in rats was measured using daily
weight assessment. (A) Representative images of T2-weighted (T2w) MRI scans (left panel) at 48 h post-MCAO and Nissl staining (right panel) at day 11
post-MCAO, in adult rats. T2w slice images depict infarct damage as a pale delineated area. Nissl-stained slices depict the infarcted area as an increased
intensity of staining. Infarct volume, expressed as a percentage of the ipsilateral hemisphere, measured at 48 h post-MCAO using T2w MRI and at day 11
post-MCAO through Nissl staining. Analysis performed using unpaired t-test with Welch’s corrections, within time point (n: vehicle = 5, VAS2870 = 5).
All data are expressed as mean ± standard deviation. (C) Probability of survival analysis, including severity limit excluded animals, indicates NADPH
oxidase inhibition significantly increased the probability of survival and reduced outcome severity (log rank test; *p<0.05; n: vehicle = 10, VAS2870 = 6).
(D) Weight expressed as a percentage of weight at MCAO (baseline), measured daily for 11 days post-MCAO. The arrow indicates the MCAO procedure
(two-way repeated measures analysis of variance with Sidak’s multiple comparisons, assessing both between and within treatment group differences
(**p<0.01; n: vehicle = 6, VAS2870 = 6). All data are expressed as mean ± standard deviation.
Abbreviations: Contra: Contralateral hemisphere; Ipsi: Ipsilateral hemisphere; MCAO: Middle cerebral artery occlusion; MRI: Magnetic resonance
imaging; NADPH: Nicotinamide adenine dinucleotide phosphate.

Volume 11 Issue 4 (2025) 81 doi: 10.36922/jctr.25.00018