Page 52 - TD-1-1

Tumor Discovery Dual-targeting of cancer receptors by nanoparticles

Figure 1. Schematic view of different steps of synthesis. Binding of HA and FA to Fe O -DPN was done through amide bonds. TMX was loaded onto
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nanocarrier through electrostatic bonds.
acetone and separated using the DynaMag TM-50 system were transferred to flask B and stirred for 24 h. Finally, the
(yield was 76.74%). reaction mixture was washed with ether, and the products
were separated using the DynaMag TM-50 system (yield
2.5. Synthesis of Fe O -DPN-HA-FA
3 4 was 90.81%).
To activate FA, 48.55 mg (0.11 mmol) of it was dissolved
in 10 mL of dimethyl sulfoxide (DMSO), and then, DCC 2.6. Loading of TMX
(27.2 mg) and NHS (15.19 mg) were added to the reaction In the first step, 50 mg TMX was dissolved in 2 mL
mixture and stirred overnight in argon atmosphere. DMSO and 9 mL phosphate-buffered saline (PBS),
Triethylamine (500 µL) was used to shift pH into the and then, 1 mL of NaCl (20 mM) solution was added
alkaline range. After that, 40 mL of hexane was added to the dropwise to the solution and stirred for 10 min. After
reaction mixture at 0°C and precipitated by centrifugation reading the absorption of the initial solution of TMX at
at 8000×g. The yellow product was washed with ether 278 nm, Fe O -DPN-HA-FA NPs (130 mg) were added
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(yield was 70.59%). to the reaction mixture and sonicated for 15 min, then
To synthesize Fe O -DPN-HA-FA (compound 5 shown kept under shaking (170 rpm) at 25°C for 48 h. Finally,
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in Figure 1), 9.8 mL of activated FA was dissolved in 2 mL the reaction mixture was transferred to a 50 mL Falcon
of DMSO (flask A). Separately, all products of the previous and stored at 4°C for 2 h, and then, the Fe O -DPN-HA-
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step (Fe O -DPN-HA-NH NPs) dispersed to 5 mL of FA-TMX NPs (compound 6 shown in Figure 1) were
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DMSO at 40°C (flask B). Thereupon, the contents of flask A collected from the solution by an external magnetic field
Volume 1 Issue 1 (2022) 3 https://doi.org/10.36922/td.v1i1.41