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Tumor Discovery MMP-1 as a potential biomarker for gastric cancer

A B

Figure 3. Effect of MMP-1 overexpression on the proliferative ability of gastric cancer cells: (A) Western blot validation of the upregulation of MMP-1
expression; (B) Percentage of EdU-positive cells.
Note: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001
Abbreviations: Ad-MMP-1: MMP-1-overexpression plasmid; NC: Negative control; MMP-1: Matrix metalloproteinase-1.

A B

Figure 4. Effect of MMP-1 disruption on the proliferative ability of gastric cancer cells: (A) Western blot validation of MMP-1 disruption and (B) statistics
of the percentage of EdU-positive cells.
Note: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001
Abbreviation: NC: Negative control; MMP-1: Matrix metalloproteinase-1.

3.4. Effect of MMP-1 on the epithelial-mesenchymal barrier in tumor cells. These enzymes are essential for
transition (EMT)-related protein expression in tumor infiltration and metastasis. Moreover, MMPs such
gastric cancer cells as MMP-1, MMP-2, and MMP-9 20-22 are involved in tumor
Analysis of the MMP-1 effect on EMT-related protein EMT by interacting with each other. 9,23
expression in gastric cancer cells revealed significantly Differentially expressed genes were identified using
increased vimentin expression, whereas a lower expression BioGRID, STRING, GO, and KEGG pathway analyses,
of E-cadherin was observed with the overexpression revealing that MMP-1 expression was considerably
of MMP-1. Similarly, following the downregulation of upregulated in gastric cancer tissues and cells, as well as in
MMP-1, a significant decrease in vimentin expression and clinical serum samples, based on tissue protein assays. 23-27
an increase in E-cadherin expression were observed in Similar findings were reported for esophageal carcinoma,
N87 cells (Figure 6). demonstrating that MMP-1 functions as an oncogene
and the high expression of MMP-1 in esophageal cancer
4. Discussion was closely associated with lymph node metastasis,
Matrix metalloproteinases are secreted by many cells and microvascular density, and advanced TNM (tumor, node,
are involved in various physiological processes, such as and metastasis) staging. 28-30 A combination of 19 serum
11
10
angiogenesis and embryogenesis. They also play a role proteins was reported to act as a diagnostic marker to
in many pathological conditions, including myocardial distinguish patients with TNM Stages I-II, with MMP-1
infarction, fibrotic diseases, osteoarthritis, 14,15 and being the best in terms of performance. These outcomes
13
12
cancer. 16,17 At least 11 MMPs function within cells, suggest that MMP-1 could be a potential biomarker for
including MMP-1, MMP-3, MMP-7, MMP-8, MMP-9, the early diagnosis and prevention of gastric cancer.
31
MMP-10, MMP-11, MMP-12, MMP-14, MMP-23, and In colorectal cancer, in vivo and ex vivo experiments
MMP-26. 18,19 MMPs can disrupt the histologically observed confirmed that the expression of MMP-1 was higher than
Volume 3 Issue 1 (2024) 5 https://doi.org/10.36922/td.1973