Page 89 - TD-3-1

Tumor Discovery

ORIGINAL RESEARCH ARTICLE
Matrix metalloproteinase-1 as a potential

biomarker for early gastric cancer detection and
its effect on gastric cancer cell proliferation and

migration

1†
1†
2
Ke Yi , Yan Hu , Xiaoli Zhu , and Qing Li *
2†
1 Central Laboratory, The First People’s Hospital of Taicang, Soochow Medical College of Soochow
University, Taicang Affiliated Hospital of Soochow University, Suzhou, China
2 Department of Gastroenterology, The First People’s Hospital of Taicang, Soochow Medical College
of Soochow University, Taicang Affiliated Hospital of Soochow University, Suzhou, China

Abstract
The present study aimed to investigate the association between matrix
metalloproteinase-1 (MMP-1) and early gastric cancer (EGC), while also evaluating
the effect of MMP-1 on gastric cancer cell proliferation and migration. Transcriptome
RNA sequencing and database analysis were conducted to assess the relationship
between MMP-1 expression and EGC. Differences in MMP-1 expression between
clinical EGC samples and paracancerous tissues were detected using fluorescence
quantitative polymerase chain reaction (PCR). In N87 gastric cancer cells, changes
† These authors contributed equally in proliferation- and migration-related indicator expression were determined. Gene
to this work. sequencing revealed differential expression of MMP-1 in early and advanced gastric
*Corresponding author: cancers. Furthermore, enhanced MMP-1 expression was observed in early and
Qing Li advanced gastric cancer tissues, exhibiting a positive correlation with the malignant
(liqing66878@suda.edu.cn)
phenotype in gastric cancer cell lines. Fluorescence quantitative PCR revealed
Citation: Yi K, Hu Y, Zhu X, Li Q. considerably higher MMP-1 expression in EGC tissues than in paracancerous tissues.
Matrix metalloproteinase-1 as a
potential biomarker for early gastric CCK8 and EdU assays demonstrated a significant increase in N87 cell proliferation on
cancer detection and its effect on MMP-1 upregulation and a decrease on its downregulation. The scratch assay results
gastric cancer cell proliferation demonstrated a corresponding enhancement in N87 cell migratory capacity with
and migration. Tumor Discov.
2024;3(1):1973. MMP-1 upregulation, which was attenuated on its downregulation. Western blot
https://doi.org/10.36922/td.1973 experiments revealed a decrease in the expression of the epithelial-mesenchymal
transition-related protein E-cadherin after MMP-1 upregulation, while vimentin
Received: September 28, 2023
Accepted: January 12, 2024 expression significantly increased. Conversely, the downregulation of MMP-1
Published Online: March 26, 2024 led to opposite outcomes. Overall, MMP-1 emerges as a potential biomarker for
Copyright: © 2024 Author(s). EGC diagnosis and plays a crucial role in the regulation of N87 gastric cancer cell
This is an Open-Access article proliferation and migration.
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, Keywords: Proliferation; Gastric cancer; MMP-1; Early diagnosis; Migration
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with 1. Introduction
regard to jurisdictional claims in
published maps and institutional Gastric cancer is among the most prevalent cancers worldwide, ranking as the third most
affiliations. common cancer after breast and lung cancers, and represents the second leading cause of

Volume 3 Issue 1 (2024) 1 https://doi.org/10.36922/td.1973