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Tumor Discovery                                        Somatic mutations in POLE and POLD1 in colorectal cancer


















            Figure A1.  POLE inner primer specificity testing.  Gel electrophoresis   Figure A2. POLD1 inner primer specificity testing. Gel electrophoresis
            shows specific products generated by PCR using inner primers for all   showing specific products generated by PCR using inner primers for
            the  POLE  exons  examined  in  this  study.  Lane  1:  exon  9;  lane  2:  exon   all the  POLD1 exons examined in this study. Lanes 1 and 2: exon 8;
            10; lane 3: exon 11; lane 4: exon 12; lane 5: exon 13; lane 6: exon 14.   lane 3: exon 9; lane 4: exon 10; lane 5: exon 11; lane 6: exon 12; lanes
            Notes: M: DNA ladder; -ve: negative control.       7 and 8: exon 13. Notes: M: DNA ladder; -ve: negative control.




























































            Volume 3 Issue 3 (2024)                         11                                doi: 10.36922/td.3659
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