Page 77 - TD-3-3

Tumor Discovery

ORIGINAL RESEARCH ARTICLE
Effect and mechanism of the PTMAP5–hsa-miR-

22-3p–KIF2C regulatory axis on the occurrence
and development of hepatocellular carcinoma

Qing Deng 1 , Yuanchao Wei 1 , Jiali Meng 1 , and Xiaolong Li *
2
1 Department of Clinical Medicine, Faculty of First Clinical Medical College, Guangxi Medical
University, Nanning, Guangxi, China
2 Department of Cell Biology and Genetics, School of Preclinical Medicine, Key Laboratory of
Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Medical University,
Nanning, Guangxi, China

Abstract

Hepatocellular carcinoma (HCC), a primary liver cancer, is known for its high
malignancy potential. Despite extensive research, the etiology of HCC remains elusive,
with numerous molecular mechanisms yet to be deciphered. This study delves into
the influence and mechanism of the PTMAP5–hsa-miR-22-3p–KIF2C regulatory axis
in HCC pathogenesis. A comparative analysis of gene expression profiles between the
GSE87630 and GSE45267 datasets unveiled a cohort of 346 differentially expressed
genes. Protein–protein interaction networks were established using the STRING
database, and key genes were identified through receiver operating characteristic
*Corresponding author: curve analysis and LASSO regression analysis. The expression and prognostic value
Xiaolong Li of these key genes were further evaluated using GEPIA and Kaplan–Meier analysis.
(xlongli@outlook.com) Upstream miRNAs were predicted using the MiRTarBase and StarBase databases,
Citation: Deng Q, Wei Y, Meng J, facilitating the construction of the PTMAP5–hsa-miR-22-3p–KIF2C regulatory axis.
Li X. Effect and mechanism of the Co-expression analysis of KIF2C was performed through UALCAN and StarBase, and
PTMAP5–hsa-miR-22-3p–KIF2C
regulatory axis on the occurrence the regulatory axis was found to play a pivotal role in HCC development through
and development of hepatocellular the cell cycle, oocyte meiosis, and FOXO signaling, as revealed by DAVID enrichment
carcinoma. Tumor Discov. analysis. Furthermore, the expression and prognostic significance of KIF2C in various
2024;3(3):2846.
doi: 10.36922/td.2846 cancer types were assessed using TIMER and GEPIA, while TISIDB analysis revealed
correlations between KIF2C expression and relevant major histocompatibility complex
Received: January 30, 2024 molecules, immunomodulators, chemokines, receptors, and immune variants in
Accepted: July 10, 2024 HCC. These findings also extended to HCC molecular subtypes. In conclusion, we
Published Online: September 24, constructed a novel PTMAP5–hsa-miR-22-3p–KIF2C regulatory subnetwork, which
2024 could provide valuable therapeutic targets and diagnostic markers for HCC.
Copyright: © 2024 Author(s).
This is an Open-Access article Keywords: PTMAP5–hsa-miR-22-3p–KIF2C regulatory axis; Hepatocellular carcinoma;
distributed under the terms of the
Creative Commons Attribution Bioinformatics; Receiver operating characteristic curve analysis; LASSO regression
License, permitting distribution, analysis
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience 1. Introduction
Publishing remains neutral with
regard to jurisdictional claims in Globally, liver cancer ranks as the sixth most common cancer and the third leading cause
published maps and institutional
affiliations. of cancer-related deaths, with hepatocellular carcinoma (HCC) being the predominant

Volume 3 Issue 3 (2024) 1 doi: 10.36922/td.2846