Page 253 - EJMO-9-2

Eurasian Journal of
Medicine and Oncology Prognosis of colon adenocarcinoma

CHGA expression than the general population. cAMP- normal colon cell line, NCM460. They discovered that
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dependent protein kinase inhibitor-beta is a type of protein all colorectal cancer cells exhibited higher calcium ion
kinase inhibitory peptide. It has been shown to be highly concentrations than the normal cell line. Furthermore,
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expressed in colorectal cancer tissues. In addition, a study Pan and Xie reported that calcium ion-focal adhesion
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that compared the expression levels of PKIB in these kinase signaling activation enhanced FOXC2 mRNA
tissues with those of matching normal tissues found that stability, promoting colorectal cell invasion, migration,
it positively correlated with T-stage and poor prognosis in and proliferation. 28
colorectal cancer patients. 24
We integrated risk subtypes and clinical features to 5. Conclusion
predict the prognosis of each COAD patient accurately. In this study, we developed a robust eight-gene signature
While age and gender did not significantly differ between and a corresponding column line graph to predict the
high- and low-risk groups, significant differences were prognosis of colorectal cancer patients. This tool could
observed in T, N, and M staging, as well as clinical staging, assist in identifying high-risk individuals who may benefit
suggesting that these criteria could be crucial clinical from more intensive treatment strategies.
traits in identifying risk subtypes. We discovered that the
eight-mRNA signature was an independent predictive Acknowledgments
factor after incorporating these clinical characteristics The authors would like to thank Tianjin University
and risk signatures into univariate and multifactorial of Traditional Chinese Medicine for supporting the
Cox regression analysis in the TCGA and GEO datasets. experiment.
High-risk patients were more likely to develop resistance
to xeloda, camptothecin-11, and L-OHP than low-risk Funding
patients. However, there were no significant differences This study was supported by the Tianjin Municipal Health
in resistance between high- and low-risk groups for Commission Chinese Medicine and Western Medicine
5-fluorouracil, which may assist in optimizing drug Research Project (2023193), the Tianjin Municipal Health
dosage. Clinical characteristics and eight-mRNA profiles Commission Chinese Medicine and Western Medicine
were created as column line plots to predict each COAD Research Project (2023222), and the Tianjin Municipal
patient’s prognosis precisely. The high accuracy of our Education Commission Subjects (2022KJ187).
eight-mRNA signature for COAD prognosis was further
demonstrated by calibration curves, which displayed a Conflict of interest
high agreement between anticipated and actual observed
survival times. The authors declare no competing interest.
The prognosis of patients with tumors is significantly Author contributions
influenced by immune cell infiltration and immunological
activities. With significant differences in immune cell Conceptualization: Yuying Yang, Cuiying Wang, Zhongqiu
infiltrations and immune function between high- and low- Chai
risk groups, a robust correlation between the eight-mRNA Formal analysis: Hongqian Wei, Bing Zhou, Yuying Yang,
signature and various immune profiles was discovered in Xiaochen Pang
this study. Methodology: Yuying Yang, Cuiying Wang, Zhongqiu Chai,
Wenhai Dong
We selected the top five key pathways for analysis after Writing – original draft: Yuying Yang, Cuiying Wang
performing pathway enrichment analysis using GSEA, Writing – review & editing: Zhongqiu Chai, Songtao Hou
which revealed significant enrichment in the low-risk
group. Interestingly, calcium ion metabolism is implicated Ethics approval and consent to participate
in four of the five key pathways. Calcium ions, the most Not applicable.
prevalent intracellular second messenger, play a critical
role in regulating various physiological processes through Consent for publication
cell signaling. Intracellular calcium ions homeostasis is
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tightly regulated, and its imbalance has been associated Not applicable.
with tumor growth, invasion, and metastasis. A study Availability of data
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by Tsoi et al. compared calcium ion concentrations in
colon cancer cell lines (caco-2, DLD-1, HCT116, LoVo, The data analyzed in this study are available in the TCGA
HT29, LS180, SW480, SW620, and SW1116) and the and GEO databases.

Volume 9 Issue 2 (2025) 245 doi: 10.36922/EJMO025060024