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Global Translational Medicine Proteomic analysis of heart in metabolic cardiomyopathy

A B

Figure 6. Kyoto Encyclopedia of Genes and Genomes analysis of high-fat diet group-specific differentially expressed proteins. Annotated differentially
expressed proteins were distributed in nine protein classes (A) and seven pathways (B).

A A B

B

Figure 8. Western blot analysis of CPT1B and ACAA2. CPT1B (A) and
ACAA2 (B) in cardiomyocytes from indicated mice were detected by
Western blot. CPT1B and ACAA2 were incubated with corresponding
antibodies and tubulin, which was used as reference. Quantification was
performed by imageJ.

mechanisms and potential treatment alternatives for
MC. The specific molecular mechanism of metabolic
Figure 7. Immunostaining of CPT1B and ACAA2. CPT1B (A) and
ACAA2 (B) in cardiomyocytes from indicated mice were detected by dysfunction in MC needs to be further studied.
double labeling immunofluorescence. CPT1B and ACAA2 were labeled
by corresponding antibodies (red) and DAPI labeled nucleus (blue). Acknowledgments
We would like to acknowledge Li Rong and Zhang Qi for
5. Conclusion their supports in data analysis and mass spectrum.
Following the comparison of DEPs between samples from Funding
the control and HFD groups, we revealed novel DEPs This work was supported by grants from the key
between normal and MC mice. This finding provided projects of Sichuan Science and Technology Department
essential clues for understanding the specific pathogenetic (No. 2019YFS0537) and the Doctoral Research Initiation

Volume 1 Issue 2 (2022) 8 https://doi.org/10.36922/gtm.v1i2.137