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Global Translational Medicine ECM receptor pathway in endotheliocytes after MI

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Figure 1. The myocardial infarction model was successfully established. (A) The representative map shows triphenyl tetrazolium chloride staining of heart
sections, with red indicating the non-infarction area and white indicating the infarcted area. (B) Representative images of collagen deposition areas stained
with Sirius Red, where red represents the collagen deposition area. (C) Statistical chart for IS/LV% (34.06 ± 3.19% [n = 6] vs. 5.59 ± 1.19% [n = 3]; P < 0.05)
and CVF% (39.8 ± 7.16% vs. 2.78 ± 1.23%, n = 6, P < 0.05).

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Figure 2. TMT proteomics detection of primary endothelial cells. (A) Flow chart of the methodology. (B) Verification of endothelial cell purity by flow
cytometry. (C) Overview of differential protein detection by TMT proteomics. (D) Differential protein volcanic map.
Abbreviations: HPLC: High-performance liquid chromatography; MS: Mass spectrometry; TMT: Tandem mass tagging.

were upregulated, 138 in organelle components, and 85 in mainly exhibited binding functions, involving 131 proteins,
membrane components. Conversely, the down-regulated and catalytic activity function, with 43 proteins. On the
proteins involved 228 proteins in cell components, 224 in other hand, the down-regulated proteins were mainly
organelle components, and 116 in membrane components. associated with binding function, involving 157 proteins,
In terms of molecular functions, the up-regulated proteins and catalytic activity, involving 141 proteins.

Volume 2 Issue 4 (2023) 5 https://doi.org/10.36922/gtm.2217