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Global Translational Medicine Comparative study of Schisandra species on VCI

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Figure 5. Comparative effects of SCF and SSF on white matter integrity following BCAS surgery. (A) LFB-stained images showing increased myelin
rarefaction and white matter lesion formation in the Cc (paramedian), fimbria of the hippocampus (Fi), ventral posteromedial thalamic nucleus (VPT),
and Cpu (striatum) of BCAS mice, with a visible protective effect in the SCF- and SSF- treated groups. All images were taken under identical exposures and
conditions (Scale bar: 200 μm; magnification: 20×). (B-E) Quantification analysis of LFB-staining in Cc, Fi, VPT, and Cpu, respectively. Data are presented
as mean ± SEM (n = 3 per group). Notes: *P < 0.05 and **P < 0.01, compared with the BCAS group (MOD); P < 0.05 and P < 0.01, compared with the
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control group (CON).
Abbreviations: POS: Positive control; SCF: Schisandra chinensis; SSF: Schisandra sphenanthera; BCAS: Bilateral common carotid artery stenosis; Cc:
Corpus callosum; Cpu: Caudate putamen.
lignans, 4 furofurans, 2 lignan lactones), 10 carboxylic 3.7. Pharmacokinetic differences between
acids (5 eicosanoids, 5 carboxylic acids), 2 lactones, 2 compounds in SCF and SSF
sesterterpenoids, and 1 pyrrilium (Table S1). For SSF, a Compounds with high bioavailability and drug-likeness
total of 63 compounds were identified, including 21 lignans properties are more likely to be absorbed. Bioactive compounds
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(9 diphenylcyclooctene lignans, 4 dibenzylbutane lignans, with therapeutic effects are generally expected to exhibit passive
5 tetrahydrofuran lignans, 2 aryltetralin lignans, 1 furanoid HIA properties. Therefore, we further conducted analysis of
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lignan), 2 terpenoids (1 triterpenoid, 1 sesquiterpenoid), six physicochemical properties, HIA and BBB permeation, and
1 lactone, 17 phenylpropanoids (16 phenylpropanoids, 1 interactions with P-gp in the identified compounds.
flavonoid), 1 phenol, 16 alkaloids, 4 carboxylic acids, and As shown in Figure 8, the predicted lipophilicity, size,
1 sulfoxide (Table S2). These results suggest that SCF and polarity, solubility, flexibility, and saturation properties
SSF exhibit distinct chemical compositions, which may were evaluated for the identified compounds. A total of
contribute to different pharmacological effects. 64 compounds in SCF and 60 compounds in SSF were

Volume 4 Issue 1 (2025) 97 doi: 10.36922/gtm.6879