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International Journal of AI
            for Material and Design                                               ML in 3D bioprinting of cultivated meat




            Table 3. Summary of published works on the prediction of total viable count in beef
            Foods    ML method   Analytical   Sample size Details              Performance           References
                                 technique
            Raw beef  Supervised:   FTIR      74    Inputs:                    90.5% overall accuracy   75
                     MLP                             •  Top five PCs from FTIR spectral data  91.7% accuracy for
                                                      in the range of 1800 – 1000 cm-1   fresh samples
                                                    Outputs:                   81.3% accuracy for
                                                     • TVC classification into three bins  semi-fresh samples
                                                    MLP model details:         94.1% accuracy for spoiled
                                                     • One hidden layer with 10 nodes  samples
                                                    Logistic sigmoid transfer function  RMSE = 0.85, SEP = 12.94%
            Raw beef  Supervised:   E-nose    12    Inputs:                    93.73% accuracy          76
                     MLP                             • E‑nose data from 11 gas sensors
                                                    Outputs:
                                                     • TVC classification into four bins
                                                    BPNN Model details:
                                                     • Two hidden layers, 32 and 12 nodes
                                                    Leaky ReLU activation function
            Raw beef  Supervised:   Raman     20    Inputs:                    R  = 0.90, RMSEP = 0.38 for   77
                                                                                2
                     PLSR        Spectroscopy       •  Raman spectral data in the range    TVC
                                                     of 1800 – 500 cm-1        R  = 0.75, RMSEP = 0.60 for
                                                                                2
                                                    Outputs:                   LAB
                                                     • TVC value or LAB count
            Raw beef  Supervised:   MSI       85    Inputs:                    i. PLS-DA:               78
                     PLS-DA PLSR                     •  First five LVs from MSI in the range   80.0% accuracy for
                                                      of 405 –970 nm            TVC classification
                                                    Outputs:                   PLSR:
                                                     •  PLS‑DA: TVC classification into   r = 0.78, RMSEP = 1.29 for
                                                      three bins                TVC
                                                     •  PLSR: TVC, Pseudomonas spp., and   r = 0.84, RMSEP = 1.12 for
                                                      B. thermosphacta          Pseudomonas spp.
                                                                                r = 0.86, RMSEP = 1.00 for
                                                                                B. thermosphacta
            Raw beef  Supervised: SVR MSI     164   Inputs:                    80.8% accuracy           79
                                                                                2
                                                     •  18 wavelengths from MSI in the   R  = 0.98, RMSEP = 0.14
                                                      range of 405 – 970 nm
                                                    Outputs:
                                                     • TVC classification into two bins
                                                    SVR Model details:
                                                     • Radial basis function kernel
            Cooked beef Supervised:   his     105   Inputs:                    PLSR:                    80
                     PLSR SVM                        •  Full spectrum from HSI data in the   Full spectrum:
                                                      range of 400 – 1000 nm    88.57% accuracy
                                                     •  15 wavelengths selected by random   Random frog:
                                                      frog from HSI data in the range of   88.57% accuracy
                                                      400 – 1000 nm             Correlation analysis:
                                                     •  Six wavelengths selected by Random   85.71% accuracy
                                                      Frog, then Correlation Analysis from  SVM:
                                                      HSI data in the range of    Full spectrum:
                                                      400 – 1000 nm             91.43% accuracy
                                                    Outputs:                    Random frog:
                                                     • TVC classification into three bins  91.43% accuracy
                                                                                Correlation analysis:
                                                                                97.14% accuracy
            Abbreviations: BPNN: Backpropagation neural network; FTIR: Fourier Transform Infrared Spectroscopy; HSI: Hyperspectral imaging; LAB: Lactic
            Acid Bacteria; MLP: Multilayer perceptron; MSI: Multispectral imaging; PLS-DA: Partial least squares discriminant analysis; PLSR: Partial least
            squares regression; ReLU: Rectified linear unit; RMSE: Root-mean squared error; RMSEP: Root-mean squared error of prediction; SEP: Standard
            error of prediction; SVM: Support vector machine; SVR: Support vector regression; TVC: Total viable count; Brochothrix thermosphacta: Brochothrix
            thermosphacta.



            Volume 1 Issue 1 (2024)                         16                      https://doi.org/10.36922/ijamd.2279
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