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International Journal of AI
            for Material and Design                                               ML in 3D bioprinting of cultivated meat




            Table 2. (Continued)
            Foods   ML method  Analytical   Sample size Details            Performance               References
                               technique
                                                  •  wavelet transform from 300×300   R  = 0.52 for off-flavor
                                                                             2
                                                   pixels                    R  < 0.01 for overall liking
                                                                             2
                                                  •  LD1, LD2, and LD3 accounted for   ii. BPNN:
                                                   96.5% of the variance     R  = 0.99 for brownness
                                                                             2
                                                  BPNN Model details:        R  = 0.93 for texture clarity
                                                                             2
                                                    •  33 input nodes for 33 features   R  = 0.95 for chewiness
                                                                             2
                                                     from E-nose, e-Tongue, and   R  = 0.99 for fibrousness
                                                                             2
                                                     computer vision data    R  = 0.98 for hardness
                                                                             2
                                                    • One hidden layer with 45 nodes  R  = 0.99 for juiciness
                                                                             2
                                                    • Nine output nodes      R  = 0.89 for meaty odor
                                                                             2
                                                                             R  = 0.97 for off-flavor
                                                                             2
                                                                             R  = 0.75 for overall liking
                                                                             2
            Abbreviations: BPNN: Backpropagation neural network; CPMG: Carr-Purcell Meiboom-Gill; CWFP: Continuous Wave-Free Precession; LD: Linear
            discriminant; LDA: Linear discriminant analysis; LV: Latent variable; MR: Nuclear magnetic resonance; NIR: Near infrared; PCA: Principal component
            analysis; PLSR: Partial least squares regression; SVM: Support vector machine; TD-NMR: Time-domain nuclear magnetic resonance;
            VIS: Visual reflectance spectroscopy.
            electrodes. PCA was used on electrode data, with the top   ranging from 0.93 to 0.99 except for R  = 0.89 for meaty
                                                                                              2
            five PCs accounting for 73.29% of the variance. These were   odors and  R  = 0.75 for overall liking. In contrast, the
                                                                         2
            then input into SVM with radial basis kernel function,   PLSR  R  values for validation ranged from 0.30 to 0.77,
                                                                     2
            achieving an accuracy of 90% in predicting beef flavor   with exceptions of R  = 0.80 for juiciness and R  < 0.01
                                                                                                       2
                                                                                2
                  65
            grading.  The study, hence, proposed that combining the   for overall liking.  The PLSR model’s performance was
                                                                             66
            sensor array with SVM analysis offers an efficient method   likely hindered by the non-linear relationship between
            for assessing the flavor characteristics of beef. While it has   the sensory attributes and the various sensors used, as it
            high prediction accuracy, it must be noted that the SVM   was designed to identify linear relationships. Although the
            classifies the prediction into five categories, while sensory   multiple-target  BPNN  model,  incorporating  fused  data
            scoring is typically conducted over a range of values.   from various sensory technologies, successfully predicted
            Support vector regression (SVR) would hence be more   sensory perception intensities with high performance
            useful for flavor prediction, but there are currently few   (R > 0.9340), it faced challenges in effectively predicting
                                                                 2
            studies specifically using SVR for meat flavor prediction.  subjective overall liking scores. This research offers valuable
              In one study, PLSR and BPNN models were employed   insights into the preparation of beef stew and quantitative
            to predict the sensory attributes of stewed beef based   sensory prediction by employing a combination of sensory
            on data from E-nose, E-tongue, and computer vision   techniques.
                66
            data.  The E-nose comprised a sensor array of 14 metal
            oxide  semiconductors.  The  E-tongue  featured  seven   4. Meat quality control
            potentiometric chemical sensors, and computer vision data   Traditional methods employed in food safety usually
            utilized discrete wavelet transform to extract 12 textural   involve numerous enrichment, culturing, incubation, and
            features from a 300 × 300-pixel region of interest. The   other preparation steps, some of which might extend over
            BPNN utilized all 33 features from the sensors as input,   a week.  Regulations governing food safety often adopt a
                                                                     67
            with one hidden layer comprising 45 nodes and an output   zero-tolerance stance toward specific pathogens such as
            layer consisting of nine nodes corresponding to sensory   Listeria monocytogenes, Shiga toxin-producing Escherichia
            attributes. The dataset comprised 30  samples in five   coli, and Salmonella,  for which the detection of individual
                                                                               68
            replicates, each yielding 33 data points from the sensors,   cells per sample may not always be guaranteed.
            and 2 replicates from a trained sensory panel evaluating
            a  total  of  28  sensory  attributes.  Despite  training  on  this   Accurate identification of microbial or chemical
            comprehensive dataset, only nine sensory attributes were   contamination through non-destructive means, such as
            predicted. Linear discriminant analysis (LDA) analysis   volatile organic compound (VOC) detection, Fourier
            revealed that the first three LDs explained 96.5% of the   transform infrared (FTIR) spectroscopy, or Raman
            variance, and the LDA plot illustrated the distinguishability   spectroscopy,  could  allow  for  larger  sample  sizes  and
            of different pressure conditions in cooking (P0, P2, P4,   establish  a more  resilient safety protocol.  Nevertheless,
            P6, and P8). The BPNN achieved R  values for validation   these techniques typically yield substantial amounts of
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            Volume 1 Issue 1 (2024)                         13                      https://doi.org/10.36922/ijamd.2279
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