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Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues

























            Figure 6. Effects of custom-made silver wire anchor. Time-dependent observations of developments of fiber-like structures of trans-
            ferred smooth muscle cells (A) without and (B) with use of the anchor. Data shown were obtained at 0 h (A-1, B-1), 24 h (A-2, B-2),
            and 36 h (A-3, B-3) after transfer onto Matrigel substrate. Scale bar = 500 µm.








































            Figure 7. Lamination by transfer printing of pre-patterned smooth muscle cells (SMCs). (A, B) The phase contrasted microscopic
            photographs of double layered structure produced by lamination via transfer printing of pre-patterned SMCs. Scale bar = 500 µm (A)
            or 200 µm (B). (C, D) Fluorescent images of double-layered structure by lamination with different stained cells. Scale bar = 200 µm
            (C), 500 µm (D).
            Link to view supplementary video.    http://dx.doi.org/10.18063/IJB.2015.01.007.V1.

            44                          International Journal of Bioprinting (2015)–Volume 1, Issue 1
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