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International Journal of Bioprinting Droplets prepared by air-focused bioprinting

to those secreted by unencapsulated CAR-T cells, Ethics approval and consent to participate
suggesting that released CAR-T cells still maintained their The cell experiments were performed with ethical
functionality. These results confirmed that encapsulation compliance and approval by The First Affiliated Hospital,
of CAR-T cells in hydrogel particles s did not compromise School of Medicine, Zhejiang University (Protocol
the cell activity and functionality but facilitated their Number: IIT20230243B).
manipulation and cell culture.

4. Conclusion Consent for publication
Not applicable.
In this work, we presented an AFMDP system for the
production of monodisperse droplets and particles with Availability of data
tunable size. The AFMDP system combined the advantages
of microfluidics and 3D printing, thus facilitating the design Data can be available for readers upon reasonable request.
of droplets, particles, and microcapsules for biomedical
applications. The microfluidic device adopted a co-axial References
flow-focusing design, which was beneficial for the stable
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Funding 5. Chen L, Yang C, Xiao Y, et al. Millifluidics, microfluidics,
This work is supported by the National Key Research and and nanofluidics: Manipulating fluids at varying length
Development Program of China (2021YFC3001101), the scales. Mater Today Nano. 2021;16:100136.
National Natural Science Foundation of China (Grant doi: 10.1016/j.mtnano.2021.100136
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The authors declare no conflicts of interest. doi: 10.1021/acs.chemrev.6b00848

Author contributions 8. Xingzheng W, Zeyong S, Dong C. Preparation of droplet
suspension by microfluidic technology. Modern Chemical
Conceptualization: Chenjing Yang, Wei Wu, Yang Gao Industry. 2020;40(9):70-74.
Investigation: Shuxing Lao, Shikai Zhang doi: 10.16606/j.cnki.issn0253-4320.2020.09.015
Methodology: Xingyu Lu 9. Gu Z, Xie M, Lv S, et al. Perfusable vessel-on-a-chip for
Writing – original draft: Chenjing Yang antiangiogenic drug screening with coaxial bioprinting. Int J
Writing – review & editing: Fangfu Ye, Peng Zhao, Bioprint. 2022;8(4):619.
Dong Chen doi: 10.18063/ijb.v8i4.619

Volume 10 Issue 1 (2024) 404 https://doi.org/10.36922/ijb.1102