Page 414 - IJB-10-1

International

Journal of Bioprinting

RESEARCH ARTICLE
PBF-LB fabrication of microgrooves for induction

of osteogenic differentiation of human
mesenchymal stem cells

Aira Matsugaki , Tadaaki Matsuzaka, Toko Mori, Mitsuka Saito, Kazuma
*
Funaoku, Riku Yamano, Ozkan Gokcekaya, Ryosuke Ozasa, and Takayoshi
Nakano *

Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University,
2-1 Yamada-oka, Suita, Osaka 565-0871, Japan

Abstract

Stem cell differentiation has important implications for biomedical device design
and tissue engineering. Recently, inherent material properties, including surface
chemistry, stiffness, and topography, have been found to influence stem cell fate.
Among these, surface topography is a key regulator of stem cells in contact with
materials. The most important aspect of ideal bone tissue engineering is to control
the organization of the bone extracellular matrix with fully differentiated osteoblasts.
Here, we found that laser powder bed fusion (PBF-LB)-fabricated grooved surface
inspired by the microstructure of bone, which induced human mesenchymal stem
*Corresponding authors: cell (hMSC) differentiation into the osteogenic lineage without any differentiation
Takayoshi Nakano supplements. The periodic grooved structure was fabricated by PBF-LB which
(nakano@mat.eng.osaka-u.ac.jp) induced cell elongation facilitated by cytoskeletal tension along the grooves. This
Aira Matsugaki resulted in the upregulation of osteogenesis via Runx2 expression. The aligned hMSCs
(matsugaki@mat.eng.osaka-u.ac.jp)
successfully differentiated into osteoblasts and further organized the bone mimetic-
Citation: Matsugaki A, Matsuzaka oriented extracellular matrix microstructure. Our results indicate that metal additive
T, Mori T, et al. PBF-LB fabrication manufacturing technology has a great advantage in controlling stem cell fate into
of microgrooves for induction of
osteogenic differentiation of human the osteogenic lineage, and in the construction of bone-mimetic microstructural
mesenchymal stem cells. Int J organization. Our findings on material-induced stem cell differentiation under
Bioprint. 2024;10(1):1425. standard cell culture conditions open new avenues for the development of medical
doi: 10.36922/ijb.1425
devices that realize the desired tissue regeneration mediated by regulated stem cell
Received: December 30, 2022 functions.
Accepted: August 8, 2023
Published Online: January 9, 2024
Copyright: © 2024 Author(s). Keywords: Additive manufacturing; Laser powder bed fusion; Mesenchymal stem
This is an Open Access article cells; Osteogenic differentiation; Bone microstructure
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium,
provided the original work is 1. Introduction
properly cited.
Publisher’s Note: AccScience Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various
1
Publishing remains neutral with tissues, including bone, fat, cartilage, muscle, and neurons. Osteogenic differentiation
regard to jurisdictional claims in of MSCs has been of interest for use as an autologous cell source, or for the development
published maps and institutional 2,3
affiliations. of medical devices in bone engineering. Tailored control of human mesenchymal
stem cells (hMSCs) differentiation is desirable in biomedical engineering and stem cell

Volume 10 Issue 1 (2024) 406 https://doi.org/10.18063/ijb.1425