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International Journal of Bioprinting                            Osteogenic differentiation of hMSCs by PBF-LB



            Author contributions                               7.   McMurray RJ, Gadegaard N, Tsimbouri PM, et al. Nanoscale
                                                                  surfaces for the long-term maintenance of mesenchymal
            Conceptualization: Aira Matsugaki, Takayoshi Nakano   stem cell phenotype and multipotency.  Nat Mater.
            Investigation: Aira Matsugaki, Toko Mori, Mitsuka Saito,   2011;10(8):637-644.
               Kazuma Funaoku, Riku Yamano, Tadaaki Matsuzaka,      doi: 10.1038/nmat3058
               Ryosuke Ozasa, Takayoshi Nakano                 8.   Dalby MJ, Gadegaard N, Tare T, et al. The control of human
            Methodology: Aira Matsugaki, Ozkan Gokcekaya,         mesenchymal cell differentiation using nanoscale symmetry
               Takayoshi Nakano                                   and disorder. Nat Mater. 2007;6(12):997-1003.
            Formal analysis:  Toko Mori, Mitsuka Saito, Kazuma      doi: 10.1038/nmat2013
               Funaoku, Riku Yamano, Tadaaki Matsuzaka, Ozkan   9.   Barradas AM, Fernandes HA, Groen N, et al. A calcium-
               Gokcekaya                                          induced signaling cascade leading to osteogenic differentiation
            Writing – original draft: Aira Matsugaki              of human bone marrow-derived mesenchymal stromal cells.
            Writing – review & editing: Takayoshi Nakano          Biomaterials. 2012;33(11):3205-3215.
                                                                  doi: 10.1016/j.biomaterials.2012.01.020
            Ethics approval and consent to participate         10.  Yang F, Yong D, Tu J, Zheng Q, Cai L, Wang L. Strontium
            Not applicable.                                       enhances osteogenic differentiation of mesenchymal stem
                                                                  cells and in vivo bone formation by activating Wnt/catenin
            Consent for publication                               signaling. Stem Cells. 2011;29(6):981-991.
                                                                  doi: 10.1002/stem.646
            Not applicable.                                    11.  Jayasree A, Raveendran NT, Guo T, Ivanovski S, Gulati K.
                                                                  Electrochemically nano-engineered titanium: Influence of
            Availability of data                                  dual micro-nanotopography of anisotropic nanopores  on
                                                                  bioactivity and antimicrobial activity.  Mater Today Adv.
            The  data  presented in  this  study  are  available  upon   2022;15:100256.
            reasonable request from the corresponding author.     doi: 10.1016/j.mtadv.2022.100256

            References                                         12.  Luo J, Walker M, Xiao Y, Donnelly H, Dalby MJ, Salmeron-
                                                                  Sanchez  M.  The  influence  of  nanotopography  on  cell
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               doi: 10.1038/s41536-019-0083-6                  13.  Matsugaki A, Aramoto G, Ninomiya T, et al. Abnormal
                                                                  arrangement of a collagen/apatite extracellular matrix
            2.   Grotheer V, Skrynecki N, Oezel L, Windolf J, Grassmann J.   orthogonal to osteoblast alignment is constructed by a nanoscale
               Osteogenic differentiation of human mesenchymal stromal   periodic surface structure. Biomaterials. 2015. 37:134-143.
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            3.   Mazzoni E, Mazziotta C, Iaquinta  MR, et al. Enhanced      doi: 10.1007/s11665-014-0958-z
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               mesenchymal stem cells by a hybrid hydroxylapatite/  15.  Hagihara K, Nakano T. Control of anisotropic
               collagen scaffold. Front Cell Dev Biol. 2021;8:610570.    crystallographic texture in powder bed fusion additive
               doi: 10.3389/fcell.2020.610570                     manufacturing of metals and ceramics - A review. J Metals.
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               doi: 10.1038/nmat3937                           16.  Matsugaki A, Aramoto G, Nakano T. The alignment of
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            5.   Engler AJ, Sen S, Sweeney HL, Discher DE. Matrix elasticity   dislocation motion. Biomaterials. 2012;33(30):7327-7335.
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            6.   Park JS, Chu JS, Tsou AD, et al. The effect of matrix stiffness   matrix structure depends on the degree of osteoblast
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            Volume 10 Issue 1 (2024)                       414                        https://doi.org/10.18063/ijb.1425
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