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Utilising inkjet printed paraffin wax for cell patterning applications

Figure 7. Micrographs showing (A) fibroblasts aligning within wax structures on glass, after 2 days of cell culture on wax structures,
(B) immediately after wax removal, (C) 24 hours and (D) 48 hours after wax removal taken in the same sample region of interest.
Each image was processed with OrientationJ to highlight the alignment of cells within the channel. Bar = 50 µm.

irregular surfaces as a substrate. http://dx.doi.org/10.1126/science.276.5317.1425
Future research will involve the application of pat- 4. Dike L E, Chen C S, Mrksich M, et al., 1999, Geometric
terned environments with co-culture of cells. Orga- control of switching between growth, apoptosis, and
nised formations of cell constructs can be created differentiation during angiogenesis using micropatterned
which would be of benefit to fields such as neuronal substrates. In Vitro Cellular & Developmental Biolo-
research. Nerve cells could be patterned onto a sample gy-Animal, vol.35(8): 441–448.
within an organised wax structure, and once the nerve http://dx.doi.org/10.1007/s11626-999-0050-4
cells have adhered and the wax has been removed, 5. McBeath R, Pirone D M, Nelson C M, et al., 2004, Cell
support cells can be seeded on the sample to create a shape, cytoskeletal tension, and RhoA regulate stem cell
patterned co-culture environment. lineage commitment. Developmental Cell, vol.6(4):
483–495.
Conflict of Interest and Funding http://dx.doi.org/10.1016/S1534-5807(04)00075-9
6. Banerjee S, Sknepnek R and Marchetti M C, 2014, Op-
No conflict of interest was reported by the authors. timal shapes and stresses of adherent cells on patterned

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