Page 55 - IJB-2-1

Elizabeth V. Koudan, Elena A. Bulanova, Frederico DAS Pereira, et al

sian Research Fund (project number 15-15-00173).

References

1. Derby B, 2012, Printing and prototyping of tissues and
scaffolds. Science, vol.338(6109): 921–926.
http://dx.doi.org/10.1126/science.1226340
2. Murphy S V and Atala A, 2014, 3D bioprinting of tissues
and organs. Nature Biotechnology, vol.32(8): 773–785.
http://dx.doi.org/10.1038/nbt.2958
3. Atala A and Yoo J J, 2015, Essentials of 3D Biofabrica-
tion and Translation, Academic Press, United States.
4. Chua C K and Yeong W Y, 2015, Bioprinting: Principles
and Applications, World Scientific Publishing Company,

Figure 12. Tissue constructs formed by attachment of patterned Singapore.
tissue spheroids to electrospun polyurethane matrix as a tech- 5. Gao G and Cui X, 2015, Three-dimensional bioprinting in
nology platform for 3D bioprinting: (A) Patterned tissue sphe- tissue engineering and regenerative medicine. Biotech-
roids attached to electrospun polyurethane matrix; (B) Biofa- nology Letters, vol.37(12): 1–9.
brication of acellular collagen patches; (C) Biofabrication of http://dx.doi.org/10.1007/s10529-015-1975-1
human skin; (D) Biofabrication of cartilage. 6. Ozbolat IT, 2015, Bioprinting scale-up tissue and organ
constructs for transplantation. Trends in Biotechnology,
and tissue spheroids from keratinocytes on another vol.33(7): 395–400.
side of matrix will enable biofabrication of human skin http://dx.doi.org/10.1016/j.tibtech.2015.04.005
(Figure 12C). Using several layers of chondrospheres 7. Mironov V, Kasyanov V, Drake C, et al., 2008, Organ
attached to electrospun polyurethane matrix will also printing: Promises and challenges. Regenerative Medi-
allow to biofabricate human cartilage (Figure 12D). cine, vol.3(1): 93–103.
Finally, using magnetically functionalized electro- http://dx.doi.org/10.2217/17460751.3.1.93
spun matrices with magnetic nanoparticles [23] as well 8. Mironov V, Visconti R P, Kasyanov V, et al., 2009, Organ
as using tissue spheroids biofabricated from cells labe- printing: Tissue spheroids as building blocks. Biomate-
lled with magnetic nanoparticles [24–27] will enable the rials, vol.30(12): 2164–2174.
development of novel magnetic 3D bioprinting tech- http://dx.doi.org/10.1016/j.biomaterials.2008.12.084
nology based on principles of magnetic levitation or 9. Perez-Pomares J M and Foty R A, 2006, Tissue fusion
translocation of tissue constructs using magnetic for- and cell sorting in embryonic development and disease:
ces [28–30] . Biomedical implications. BioEssays, vol.28(8): 809–821.
http://dx.doi.org/10.1002/bies.20442
5. Conclusion 10. Hajdu Z, Mironov V, Mehesz A N, et al., 2010, Tissue
spheroid fusion-based in vitro screening assays for analy-
Tissue spheroids biofabricated from human fibroblasts sis of tissue maturation. Journal of Tissue Engineering
have been placed in regular patterns on the surface of and Regenerative Medicine, vol.4(8): 659–664.
electrospun polyurethane matrix using 3D bioprinter. http://dx.doi.org/10.1002/term.291
Spreading of patterned tissue spheroids demonstrated 11. Huang G S, Tseng C S, Linju Yen B, et al., 2013, Solid
an in vitro biocompatibiity of electrospun microfibr- freeform-fabricated scaffolds designed to carry multicel-
ous polyurethane. The biocompatible electrospun poly- lular mesenchymal stem cell spheroids for cartilage re-
urethane matrix could serve as a carrier for tissue sph- generation. European Cells and Materials, vol.26: 179–
eroids. Thus, tissue spheroids spread on microfibrous 194; discussion 194.
electrospun polyurethane matrix is a novel technolo- 12. Ozbolat IT and Yu Y, 2013, Bioprinting toward organ
gical platform for advancing biofabrication and 3D fabrication: Challenges and future trends. IEEE Transac-
bioprinting. tions on Biomedical Engineering, vol.60(3): 691–699.
http://dx.doi.org/10.1109/TBME.2013.2243912
Conflict of Interest and Funding 13. Schon B S, Schrobback K, van der Ven M, et al., 2012,
Validation of a high-throughput microtissue fabrication
No conflict of interest was reported by the authors. process for 3D assembly of tissue engineered cartilage
This research has been supported by grant from Rus- constructs. Cell and Tissue Research, vol.347(3): 629–642.

International Journal of Bioprinting (2016)–Volume 2, Issue 1 51