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Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Figure 4. In vitro biocompatibility experiment of EHA80 woodpile scaffolds cultured with MG63 determined by MTT. (A) Viability
of MG63 cell cultures on acrylic-acid coated woodpile scaffold (Group 1) and non-coated woodpile scaffolds (Group 2) shown as a
plot of absorbance against time (error bars represent 95% confidence level). (B) Micrographs of acrylic-acid coated PolyHIPE wood-
pile scaffolds stained with DAPI and Phalloidin-FITC at day 7. (C) Non-coated woodpile structure cultured with MG63 at day 7. (D)
MG63 cell spheroids formed within woodpile pores. (E) Cross sectional view of the cell spheroid.

of cell density was seeded successfully on the acrylic SEM micrographs (Figure 3C–D) where the mor-
acid coated woodpile structure and 26% for non-co- phology of the cells on acrylic acid-coated PolyHIPE
ated woodpile structures in comparison to the tissue disks are more flat compared to the rounded cell at-
culture plastic control during the initial day 0 of cell tached to the non-coated PolyHIPE disks. As shown in
seeding. Cell growth and penetration are shown in Figure 3B, immunofluorescence micrographs of the

74 International Journal of Bioprinting (2016)–Volume 2, Issue 2