Page 244 - IJB-10-2

International Journal of Bioprinting G40T60@WNT5A promotes osteoblast differentiation

3.2. Osteogenic differentiation and angiogenesis (Figure 2A and B). Based on the GO functional enrichment
may be involved in the progression of CTO&BD analysis results above, DEGs were mainly involved in
Next, functional enrichment analysis of GO and KEGG biological processes such as cell proliferation, stromal stem
was conducted for the 79 DEGs. First, the GO functional cell development, and differentiation, with activity related
analysis results indicated that 1317 DEGs were mainly to receptor ligands, cytokines, and growth factors.
enriched in BP in terms of positive regulation of endothelial
cell proliferation (GO:0001938), cell fate commitment In addition, KEGG pathway analysis revealed that
(GO:0045165), mesenchyme development (GO:0060485), DEGs were mainly enriched in signaling pathways
and mesenchymal cell differentiation (GO:0048762), such as angiogenesis, Alzheimer’s disease-presenilin
among others. In CC, they were mainly enriched in pathway, Cadherin signaling pathway, Wnt signaling
adherens junction (GO:0005912), lamellipodium pathway, and TGF-beta signaling pathway (Figure 2C).
(GO:0030027), GABA-ergic synapse (GO:0098982), and Literature reports indicate that vasculogenesis is closely
semaphorin receptor complex (GO:0002116), among related to osteogenesis, bone repair, and regeneration.
others. In MF, they are mainly enriched in receptor- Stimulating vasculogenesis could enhance the osteogenic
ligand activity (GO:0048018), signaling receptor activator differentiation potential of BMSCs, and activation of
activity (GO:0030546), cytokine activity (GO:0005125), the Wnt/β-catenin signaling pathway could promote
and growth factor activity (GO:0008083), among others osteogenic differentiation and vasculogenesis. 62-64

Figure 2. Enrichment analysis of differentially expressed genes (DEGs) in GO and KEGG terms. (A) Bubble plot showing the enrichment of DEGs in
biological process (BP, top), cellular component (CC, middle), and molecular function (MF, bottom) categories based on GO analysis. The size of the circles
denotes the number of enriched genes, and the change of color from blue to red indicates the decreasing P value. (B) Circular plot showing the enrichment
of DEGs in the main BP pathways. (C) Enrichment analysis of DEGs in KEGG pathways. The size of the circles denotes the number of enriched genes, and
the change of color from green to red indicates the decreasing P value.

Volume 10 Issue 2 (2024) 236 doi: 10.36922/ijb.1461