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International Journal of Bioprinting Stretchable scaffold for modeling fibrosis

Figure 12. Immunostaining of HCFs on PCL/GelMA_5 scaffolds with and without mechanical cyclic stimulation (i.e., [left] not stimulated and [right]
stimulated samples) for (A) α-SMA, (B) collagen III, (C) fibronectin, and (D) collagen I. Scale bar: 50 µm. Abbreviations: α-SMA: α-Smooth muscle actin;
HCFs: Human cardiac fibroblasts; PCL/GelMA_5: Poly(ε-caprolactone)-gelatin methacryloyl.

concentration, inferring better cell spreading within the their superior permeability to nutrients and metabolites.
PCL/GelMA_5 scaffold. Although all hydrogels were Moreover, cell-loaded PCL/GelMA_5 scaffolds
not cytotoxic, only GelMA_5 and GelMA_7 hydrogels mechanically stimulated with MechanoCulture T6
supported the viability of embedded cells, probably due to bioreactor reported higher expressions of proteins involved

Volume 10 Issue 3 (2024) 485 doi: 10.36922/ijb.2247