Page 67 - IJB-4-1

An nMgO containing scaffold: Antibacterial activity, degradation properties and cell responses

Figure 3. The compressive strength and compressive modulus of the PHBV/nMgO scaffolds as a function of nMgO content

(A) (B) (C)

(D) (E) (F)

Figure 4. The distribution of nMgO in PHBV matrix with (A) 0, (B) 1, (C) 3, (D) 5 and (E) 7 wt% nMgO; (F) the EDS
spectrums of point S1 and S2.

led to a significant decrease of the interfacial areas and on the PHBV scaffolds and covered almost the entire
strength between the fillers and matrix, thus resulting surface. In contrast, there was only a small amount of
in the decrease of the reinforcing efficiency. As the E. coli attaching on the PHBV/5%nMgO scaffolds. The
PHBV/5%nMgO scaffold showed optimal compressive dramatic decrease of the numbers of E. coli indicated
properties, they were selected to be further evaluated in that nMgO inhibited the adhesion and proliferation of E.
terms of antibacterial activity, degradation behaviors and coli and killed them. Moreover, it seemed the appearance
cytocompatibility. of the E. coli attached on the PHBV/nMgO scaffolds was
The adhesion and proliferation level of Escherichia abnormal, suggesting them suffered structural damage
coli (E. coli) cultured on the PHBV/5%nMgO and from the antibacterial action of nMgO (Figure 5D). The
PHBV scaffolds after 24 h were evaluated by SEM results indicated the incorporation of nMgO imparted
(Figure 5). The E. coli showed a typical rod shape. strong antibacterial activity to the scaffolds.
It was obvious that large amounts of E. coli attached As ROS production was reported to play a significant

6 International Journal of Bioprinting (2018)–Volume 4, Issue 1