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Han X, et al.

A B C

D E F

G H I

J K L

Figure 12. Fluorescence micrographs showing representative results of viability staining of 3T3 cells cultured for one day (left column),
4 days (middle column), and 14 days (right column) at samples of the reference material glass (A–C), at BLI with Irgacure 184 (D–F), at
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BLI with Irgacure 2959 (G–I), and at BLI with Irgacure 369 (J–L).
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Table 1. Levels of cytotoxicity determined by live/dead staining.

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Material Control (glass) BLI with Irgacure 184 BLI with Irgacure 2959 BLI with Irgacure 369
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Day 1 No cytotoxicity No cytotoxicity High cytotoxicity No cytotoxicity
Day 4 No cytotoxicity Low cytotoxicity; increased number High cytotoxicity Low cytotoxicity; increased number
of cells from day 1 to day 4 of cells from day 1 to day 4
Day 14 No cytotoxicity Low cytotoxicity High cytotoxicity Low cytotoxicity

International Journal of Bioprinting (2018)–Volume 4, Issue 2 11