Page 242 - IJB-10-4
P. 242
International Journal of Bioprinting N-PLN hydrogels for human skin modeling
by the expression of cellular markers, these constructs Availability of data
exhibit in vivo-like cell organization, featuring dermis and Data are available from the corresponding author upon
epidermis, and boast barrier characteristics as confirmed reasonable request.
by the TEER measurements. Cellular distribution and,
especially, epithelial-dermal cell interaction, can be References
promoted by culturing the constructs under ALI condition.
Altogether, this approach represents an alternative method 1. Moniz T, Costa Lima SA, Reis S. Human skin models: from
to develop ready-to-use skin models in an easily, fast, healthy to disease-mimetic systems; characteristics and
reproducible, and cost-affordable manner, which might be applications. Br J Pharmacol. 2020;177(19):4314-4329.
particularly beneficial for pre-clinical assays in cosmetic doi: 10.1111/bph.15184
and pharmaceutical research. 2. Dearman BL, Boyce ST, Greenwood JE. Advances in
skin tissue bioengineering and the challenges of clinical
Acknowledgments translation. Front Surg. 2021;8:640879.
Not applicable. doi: 10.3389/fsurg.2021.640879
3. Shores JT, Gabriel A, Gupta S. Skin substitutes and
Funding alternatives. Adv Skin Wound Care. 2007;20(9):493-508.
doi: 10.1097/01.ASW.0000288217.83128.f3
This work was supported by the European Union’s
Horizon Europe Research and Innovation Programme 4. Manon-Jensen T, Kjeld NG, Karsdal MA. Collagen-mediated
(B-BRIGHTER project, grant agreement no. 101057894), hemostasis. J Thromb Haemost. 2016;14(3):438-448.
doi: 10.1111/jth.13249
the Department of Research and Universities of the
Generalitat de Catalunya (2021 SGR 01495), and the 5. Sorushanova A, Delgado LM, Wu Z, et al. The collagen
CERCA Programme of the Generalitat de Catalunya. suprafamily: from biosynthesis to advanced biomaterial
N.T. acknowledges the Spanish Ministry of Science development. Adv Mater. 2019;31(1):e1801651.
doi: 10.1002/adma.201801651
and Innovation (MCIN) for her Juan de la Cierva grant
(IJC2019-040289-I, MCIN/AEI/ 10.13039/501100011033). 6. Eming SA, Martin P, Tomic-Canic M. Wound repair and
Views and opinions expressed in this work are however regeneration: Mechanisms, signaling, and translation. Sci
those of the author(s) only and do not necessarily reflect Transl Med. 2014;6(265):265sr6.
doi: 10.1126/scitranslmed.3009337
those of the European Union. Neither the European Union
nor the granting authority can be held responsible for them. 7. Silvipriya KS, Krishna Kumar K, Bhat AR, Dinesh Kumar
B, John A, Lakshmanan P. Collagen: animal sources and
Conflict of interest biomedical application. J Appl Pharm Sci. 2015;5(3):
123-127.
The authors declare no conflicts of interest. doi: 10.7324/JAPS.2015.50322
8. Schmidt FF, Nowakowski S, Kluger PJ. Improvement of
Author contributions a three-layered in vitro skin model for topical application
Conceptualization: Núria Torras, Elena Martínez of irritating substances. Front Bioeng Biotechnol.
Formal analysis: Angela Cirulli, Livia Neves Borgheti- 2020;8(May):1-11.
Cardoso, Núria Torras doi: 10.3389/fbioe.2020.00388
Investigation: Angela Cirulli, Livia Neves Borgheti-Cardoso 9. Wang H. A review of the effects of collagen treatment in
Methodology: Núria Torras, Elena Martínez, Angela Cirulli clinical studies. Polymers (Basel). 2021;13(22):3868.
Supervision: Núria Torras, Elena Martínez doi: 10.3390/polym13223868
Writing – original draft: Angela Cirulli 10. Keefe J, Wauk L, Chu S, DeLustro F. Clinical use of injectable
Writing – review & editing: Angela Cirulli, Livia Neves bovine collagen: a decade of experience. Clin Mater.
Borgheti-Cardoso, Núria Torras, Elena Martínez 1992;9(3-4):155-162.
All authors have read and approved the manuscript. doi: 10.1016/0267-6605(92)90095-b
11. Bacakova M, Pajorova J, Broz A, et al. A two-layer skin
Ethics approval and consent to participate construct consisting of a collagen hydrogel reinforced by
a fibrin-coated polylactide nanofibrous membrane. Int J
Not applicable. Nanomedicine. 2019;14:5033-5050.
doi: 10.2147/IJN.S200782
Consent for publication
12. Ackermann K, Lombardi Borgia S, Korting HC, Mewes KR,
Not applicable. Schäfer-Korting M. The Phenion® full-thickness skin model
Volume 10 Issue 4 (2024) 234 doi: 10.36922/ijb.3395

