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International Journal of Bioprinting PCL/Fe3O4@ZIF-8 for infected bone repair

Figure 3. Antibacterial properties of pure PCL, PCL/5%Fe O @ZIF-8, PCL/10%Fe O @ZIF-8, and PCL/15%Fe O @ZIF-8 scaffolds. (A, B) Representative
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pictures of colonies of S. aureus and E. coli co-cultured on different scaffolds. (C, D) Statistical analysis of colony counts of S. aureus (C) and E. coli (D)
(n = 3, ****p < 0.0001, ***p < 0.001). (E, F) Representative SEM images of S. aureus and E. coli adhesion on the surfaces of different scaffolds. The red arrows
indicate the rupture and death of bacteria (scale bars = 10 µm and 2 µm). (G, H) Statistical analysis of bacterial number of S. aureus (G) and E. coli. (H)
(n = 3, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05).
including ALP, COL-1, RUNX2, and OCN, were also ALP activity of BMSCs co-cultured with PCL/10% Fe O @
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detected by means of RT-PCR and Western blotting. ZIF-8 scaffold was also significantly higher than those with
the other scaffolds after 7 days (Figure 5B) and 14 days of
The intensity of ALP staining was stronger in BMSCs induction (Figure 5C). The quantitative results of Alizarin
co-cultured with PCL/10%Fe O @ZIF-8 scaffolds than in red staining also demonstrated that the PCL/10%Fe O @
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those with other scaffolds after both 7 days and 14 days ZIF-8 scaffold had the highest mineral deposition (Figure
of osteogenic induction (Figure 5A). The Alizarin red 5D). ALP is a marker enzyme for early osteogenic
staining after 21 days of induction showed the same trend differentiation, whereas Alizarin red staining is an
as ALP staining, with the highest staining degree of calcium approach to assessing the late-stage mineralization ability
nodules in PCL/10%Fe O @ZIF-8 group (Figure 5A). The of the scaffolds. Taken together, these results indicated that
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Volume 10 Issue 4 (2024) 306 doi: 10.36922/ijb.2271