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International Journal of Bioprinting PCL/Fe3O4@ZIF-8 for infected bone repair
Figure 5. Osteogenic differentiation of BMSCs co-cultured with different scaffolds after induction of 7, 14, and 21 days. (A) ALP staining at 7 and 14
days and Alizarin red staining at 21 days under microscope (scale bar = 200 µm; the smaller, round image at top-left corner of each staining photo is
image photographed by camera). (B−D) Statistical analysis of ALP activity after induction for 7 days (B) and 14 days (C), and semi-quantitative analysis
of Alizarin red staining (D) (n = 3, ***p < 0.001, **p < 0.01, *p < 0.05). (E−H) Gene expression of Alp (E), Runx2 (F), Col-1 (G), and Ocn (H) in BMSCs
co-cultured with different scaffolds (n = 3, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05). (I) Protein expression of RUNX2 and OCN. (J, K) Semi-
quantitative analysis of protein expression through Image J software (n = 3, ***p < 0.001, **p < 0.01, *p < 0.05).
Volume 10 Issue 4 (2024) 308 doi: 10.36922/ijb.2271
