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International Journal of Bioprinting 3D-bioprinted peripheral nerve scaffold

Figure 1. Schematic of the fabrication of the nerve conduit from induced scSHEDs and two nozzles co-bioprinting to promote nerve regeneration.
Abbreviations: GelMA: Gelatin methacrylate; PCL: Polycaprolactone; RGDAlg: arginine-glycine-aspartic acid-modified sodium alginate; scSHED:
Schwann-like stem cells from human-exfoliated deciduous teeth; SHED: Stem cells from human-exfoliated deciduous teeth.

mg of N-hydroxysulfosuccinimide (sulfo-NHS), and 16.7 medium (αMEM) (SH30265.01; HyClone, United States
mg of arginine-glycine-aspartic acid (RGD) peptide were of America [USA]). Subsequently, the hydrogel bioink
added sequentially to each gram of SA, and the mixture solution was mildly mixed with scSHEDs to reach a final
was subsequently agitated gently for 20 h at ambient concentration of 6% RGD-Alg, 5% GelMA, and 0.5% LAP,
6
temperature. The reaction mixture was then quenched with with a cell density of 2 × 10 cells/ml. This hydrogel ratio
hydroxylamine (NH OH) and dialyzed against reduced salt was selected based on our previous study. 19
2
buffer using a 3.5 kDa molecular weight cut-off (MWCO)
dialysis membrane over 5 days. The solution after dialysis 2.1.2. Thixotropic evaluation
was then subjected to sterile filtration using a 0.22 μm filter, We employed the Discovery HR2 rotational rheometer
(TA Instruments, Inc., USA) to assess the thixotropic
lyophilized, and preserved at -80℃ until the time of usage.
characteristics of the hydrogel via a three-step test at 1 Hz
Co was used to irradiate GelMA and RGD-Alg before frequency and 20℃. Initially, a shear rate of 0.1 s for 60
60
-1
configuring the bioink. A bioink solution was prepared, s was applied to stimulate the hydrogel. Subsequently, by
containing 12% w/v RGD-Alg, 10% w/v GelMA, and 1% adjusting the shear rate to 100 s and maintaining it for
-1
w/v lithium phenyl(2,4,6-trimethylbenzoyl) phosphinate 10 s, the condition of 3D bioprinting was imitated. Lastly,
(LAP) in phosphate-buffered saline (PBS). Meanwhile, the researcher reverted the shear rate to 0.1 s for another
-1
scSHEDs were subjected to enzymatic digestion with 60 s to imitate the 3D-printed scaffold. The viscosity–time
trypsin and then suspended in a minimum essential curve of the process was obtained and analyzed.

Volume 10 Issue 4 (2024) 461 doi: 10.36922/ijb.2908