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Hybrid polycaprolactone/hydrogel scaffold fabrication and in-process plasma treatment using PABS
A
B
Figure 2. (A) Set-up of the plasma-assisted bio-extrusion system; (B) system diagram: Multi-extrusion system, plasma modification system,
and control system.
distance from the bottom of the jet to the surface of 2.6. Wettability Measurement
the PCL filaments was 10 mm.
Water contact angle (WCA) measurements on the flat
2.5. Morphology Characterization surfaces of untreated and plasma-treated PCL scaffolds were
carried out with a commercial KSV contact angle measuring
Scanning electron microscopy (SEM) was used to assess the
morphology and surface characteristic of printed scaffolds. 200 systems (KSV Instruments, Finland). The system is
The scaffolds were gold/palladium coated using a Q150T equipped with a CCD video camera and a micrometric
turbo-pumped sputter coater (Quorum technologies, UK) liquid dispenser to drop 2 µL of distilled water on the surface
and imaged at 10 kV (Hitachi S3000N, Japan). These of the scaffold. The measurements of the contact angles are
images were then analyzed using ImageJ software. automatically calculated with the instrument software.
4 International Journal of Bioprinting (2019)–Volume 5, Issue 1

