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Hybrid polycaprolactone/hydrogel scaffold fabrication and in-process plasma treatment using PABS

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           Figure 2. (A) Set-up of the plasma-assisted bio-extrusion system; (B) system diagram: Multi-extrusion system, plasma modification system,
           and control system.
              distance from the bottom of the jet to the surface of   2.6. Wettability Measurement
              the PCL filaments was 10 mm.
                                                               Water  contact  angle  (WCA)  measurements  on  the  flat
           2.5. Morphology Characterization                    surfaces of untreated and plasma-treated PCL scaffolds were
                                                               carried out with a commercial KSV contact angle measuring
           Scanning electron microscopy (SEM) was used to assess the
           morphology and surface characteristic of printed scaffolds.   200  systems  (KSV  Instruments,  Finland).  The  system  is
           The scaffolds were gold/palladium coated using a Q150T   equipped with a CCD video camera and a micrometric
           turbo-pumped sputter coater (Quorum technologies, UK)   liquid dispenser to drop 2 µL of distilled water on the surface
           and imaged at 10 kV (Hitachi S3000N, Japan).  These   of the scaffold.  The measurements of the contact angles are
           images were then analyzed using ImageJ software.    automatically calculated with the instrument software.

           4                           International Journal of Bioprinting (2019)–Volume 5, Issue 1
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