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           modulus are positively correlated with the culture time   Gliosis. Glia, 50:427-34.
           and cell concentration,  because of the migration  and   8.   Anderson MF, Blomstrand  F, Blomstrand  C,  et al., 2003,
           proliferation  of astrocytes. The  construction  method  of   Astrocytes and Stroke: Networking for Survival? Neurochem
           the 3D tissue studied in this paper can be used to create   Res, 28(2):293-305. DOI 10.1023/a:1022385402197.
           in vitro glial tissue models, which can be useful for the   9.   Tatsumi K, Haga S, Matsuyoshi H,  et al., 2005,
           study of glial scar and related brain diseases.
                                                                   Characterization of Cells with Proliferative Activity after a
           Acknowledgments                                         Brain  Injury.  Neurochem  Int,  46(5):381-9.  DOI 10.1016/j.
           The work was supported by the Program of the National   neuint.2004.12.007.
           Natural Science Foundation of China [51675411] and the   10.  Norenberg MD, 1994, Astrocyte Responses to CNS Injury.
           Youth Innovation Team of Shaanxi Universities.          J Neuropathol Exp Neurol, 53(3):213-20.
                                                               11.  Gao K, Wang CR, Jiang F, et al., 2013, Traumatic Scratch
           Authors’ Contributions                                  Injury in Astrocytes Triggers Calcium Influx to Activate the

           AF and LW designed the study; AF and ZY experimented.   JNK/c-Jun/AP-1 Pathway and Switch on GFAP Expression.
           AF, LW, and RP drafted the manuscript. DC, LW, LG, XG,   Glia, 61(12):2063-77. DOI 10.1002/glia.22577.
           and JK coordinated the project and discussed the results.   12.  Sofroniew MV, 2015,  Astrogliosis.  Cold Spring Harb
           All authors read and approved the final manuscript.     Perspect Biol, 7(2):a20420.

           Conflicts of Interest                               13.  Kozai  TDY,  Jaquins-Gerstl AS,  Vazquez AL,  et al., 2015,
                                                                   Brain  Tissue  Responses to  Neural  Implants  Impact  Signal
           The authors declare that they do not have any competing   Sensitivity and Intervention Strategies. ACS Chem Neurosci,
           interests.
                                                                   6(1):48-67. DOI 10.1021/cn500256e.
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