Page 146 - IJB-10-5

International Journal of Bioprinting Liver printing: from structure to application

142 143 165 166
Bile duct channel chips enhanced bile duct system formation and liver-specific gene expression and function >4000 differentially expressed genes in the 3D co-culture model; cholangiocarcinoma cells in the 3D model were more sensitive to anti-tumor drugs Printed liver tissue enhanced ALB levels and supported capillary network formation Enhanced ALB secretion

7 days 9 days 1 week 14 days

0.03 × 0.1 × 1 mm 10 × 12 × 5 mm Cubic hepatic tissue construct thickness: ~4 mm 6 × 6 mm

HUVECs HUVECs; RBE cholangiocarcinoma cells HUVECs HUVECs; NHDFs Abbreviations: 3DP-Hos, 3D-bioprinted hepatorganoids; ADSCs, adipose-derived stem cells; ALB, albumin; dECM, decellularized extracellular matrix; DLP, digital light processing; GelMA, methacrylated gelatin; GMHA, glycidyl methacrylate-hyaluronic acid; HA, hyaluronic acid; HFFs, human foreskin fibroblasts; hiPSC, human induced pluripotent stem cell; HUVECs, human

HepaRG HepG2 HepG2 HepG2 umbilical vein endothelial cells; NHDFs, normal human dermal fibroblasts; PEGDA, polyethylene glycol diacrylate; SLA, stereolithography; UV, ultraviolet.

mentioned 33.7 ± 6.2 kPa mentioned
Not <6 kPa Not

Temperature UV light Visible light (405 nm; 20 mW/ cm 2 ) Temperature; CaCl 2 ; thrombin

dECM; gelatin Agarose; GelMA GelMA Shell: Alginate; methylcellulose Core: Collagen; fibrin; gelatin

Suspension- based bioprinting Coaxial printing

Volume 10 Issue 5 (2024) 138 doi: 10.36922/ijb.3819